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Fitness for 5 genes in
Methanococcus maripaludis S2
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500 nt
MMP_RS09070 and MMP_RS08725 are separated by 15 nucleotides
MMP_RS08725 and MMP_RS08730 are separated by 41 nucleotides
MMP_RS08730 and MMP_RS08735 are separated by 99 nucleotides
MMP_RS08735 and MMP_RS08740 are separated by 187 nucleotides
MMP_RS09070: MMP_RS09070 - F420 non-reducing hydrogenase subunit, at 1,633,982 to 1,634,116
_RS09070
MMP_RS08725: MMP_RS08725 - Ni/Fe hydrogenase subunit alpha, at 1,634,132 to 1,635,388
_RS08725
MMP_RS08730: MMP_RS08730 - F420-non-reducing hydrogenase subunit, at 1,635,430 to 1,636,296
_RS08730
MMP_RS08735: MMP_RS08735 - hydrogenase iron-sulfur subunit, at 1,636,396 to 1,636,800
_RS08735
MMP_RS08740: MMP_RS08740 - CoB--CoM heterodisulfide reductase iron-sulfur subunit A family protein, at 1,636,988 to 1,638,964
_RS08740
Group
Condition
MMP
_RS09070
MMP
_RS08725
MMP
_RS08730
MMP
_RS08735
MMP
_RS08740
nutrient t2
H2 deepwell acetate and casamino FREE
N.D.
-2.0
N.D.
N.D.
N.D.
nutrient t2
H2 deepwell casamino FREE
N.D.
-2.0
N.D.
N.D.
N.D.
nutrient t2
H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
N.D.
-2.0
N.D.
N.D.
N.D.
nutrient t2
H2 deepwell autotrophic conditions
N.D.
-1.9
N.D.
N.D.
N.D.
nutrient t2
H2 deepwell casamino FREE
N.D.
-1.9
N.D.
N.D.
N.D.
nutrient t2
H2 deepwell casamino FREE
N.D.
-1.8
N.D.
N.D.
N.D.
nutrient t2
H2 deepwell acetate and casamino FREE
N.D.
-1.7
N.D.
N.D.
N.D.
nutrient t1
Mc formate, casamino free, with 10mM L-Methionine
N.D.
-1.7
N.D.
N.D.
N.D.
nutrient t2
H2 deepwell acetate and casamino FREE
N.D.
-1.7
N.D.
N.D.
N.D.
stress
Formate 200 mM hypophosphite
N.D.
-1.7
N.D.
N.D.
N.D.
stress
McCas_H2_complete metavanadate 4.54545454545455 mM
N.D.
+1.6
N.D.
N.D.
N.D.
nitrogen source
H2 N-free with 10mM NAG
N.D.
+1.7
N.D.
N.D.
N.D.
nitrogen source
H2 N-free with 10mM Putrescine
N.D.
+1.7
N.D.
N.D.
N.D.
stress
H2 McCas with 40 minute oxygen exposure
N.D.
+1.7
N.D.
N.D.
N.D.
stress
H2 McCas with 40 minute oxygen exposure
N.D.
+1.8
N.D.
N.D.
N.D.
stress
Formate with Bacitracin 15.6 uM
N.D.
+1.8
N.D.
N.D.
N.D.
nitrogen source
H2 N-free with 5mM Inosine
N.D.
+1.8
N.D.
N.D.
N.D.
stress
McCas_H2_complete Hydroxylamine 1.04166666666667 mM
N.D.
+1.8
N.D.
N.D.
N.D.
no stress control
McCas_H2_complete no stress control
N.D.
+2.0
N.D.
N.D.
N.D.
stress
McCas_formate_complete Phenazine methosulfate 0.0333333333333333 mM
N.D.
+2.1
N.D.
N.D.
N.D.
nutrient t2
H2 deepwell vitamin FREE
N.D.
+2.1
N.D.
N.D.
N.D.
stress
Formate with Bacitracin 15.6 uM
N.D.
+2.1
N.D.
N.D.
N.D.
nitrogen source
H2 N-free with 2g/L casaminos
N.D.
+2.1
N.D.
N.D.
N.D.
nitrogen source
H2 N-free with 2g/L casaminos 10mM Ammonium chloride
N.D.
+2.1
N.D.
N.D.
N.D.
stress
McCas_H2_complete Fluorophosphate 62.5 mM
N.D.
+2.3
N.D.
N.D.
N.D.
nutrient t2
H2 deepwell acetate FREE
N.D.
+2.4
N.D.
N.D.
N.D.
stress
McCas_H2_complete Spiramycin 0.1 mM
N.D.
+2.4
N.D.
N.D.
N.D.
nutrient t2
H2 deepwell acetate FREE
N.D.
+2.5
N.D.
N.D.
N.D.
nitrogen source
H2 N-free with 2g/L casaminos 10mM Ammonium chloride
N.D.
+2.8
N.D.
N.D.
N.D.
nitrogen source
H2 N-free with 2g/L casaminos
N.D.
+2.8
N.D.
N.D.
N.D.
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