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Fitness for 5 genes in
Methanococcus maripaludis S2
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500 nt
MMP_RS08405 and MMP_RS08410 are separated by 10 nucleotides
MMP_RS08410 and MMP_RS08415 are separated by 186 nucleotides
MMP_RS08415 and MMP_RS08420 are separated by 9 nucleotides
MMP_RS08420 and MMP_RS08425 are separated by 129 nucleotides
MMP_RS08405: MMP_RS08405 - MFS transporter, at 1,577,536 to 1,578,906
_RS08405
MMP_RS08410: MMP_RS08410 - DUF169 domain-containing protein, at 1,578,917 to 1,579,609
_RS08410
MMP_RS08415: MMP_RS08415 - radical SAM protein, at 1,579,796 to 1,580,659
_RS08415
MMP_RS08420: MMP_RS08420 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,580,669 to 1,581,871
_RS08420
MMP_RS08425: MMP_RS08425 - methionine adenosyltransferase, at 1,582,001 to 1,583,218
_RS08425
Group
Condition
MMP
_RS08405
MMP
_RS08410
MMP
_RS08415
MMP
_RS08420
MMP
_RS08425
stress
McCas_H2_complete 5-Fluorouracil 0.00833333333333333 mM
-0.1
+0.4
-1.4
N.D.
N.D.
nutrient t2
H2 deepwell acetate and casamino FREE
+0.1
+0.4
-1.4
N.D.
N.D.
stress
McCas_H2_complete Cadmium chloride hemipentahydrate 0.555555555555556 mM
-0.1
+0.7
-1.0
N.D.
N.D.
nutrient t2
H2 deepwell autotrophic conditions
+0.1
+0.7
-1.3
N.D.
N.D.
nitrogen source
H2 N-free with 10mM L-Serine
-0.2
-0.6
+1.1
N.D.
N.D.
nutrient t2
H2 deepwell acetate FREE
+0.6
+0.6
-0.7
N.D.
N.D.
nutrient t2
H2 deepwell acetate and vitamin FREE
+0.5
+0.9
-0.7
N.D.
N.D.
stress
McCas_H2_complete 1,2-dimethyl-5-nitroimidazole 0.00833333333333333 mM
+0.4
+1.1
-0.9
N.D.
N.D.
stress
McCas_H2_complete metronidazole 0.00833333333333333 mM
+0.5
+1.4
-0.4
N.D.
N.D.
stress
McCas_H2_complete Benzethonium chloride 0.003125 mM
+0.2
+1.4
+0.2
N.D.
N.D.
nutrient t2
H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
+0.6
+1.0
+0.2
N.D.
N.D.
stress
H2 McCas with 1 mM Na2S
+0.5
+0.4
+0.9
N.D.
N.D.
stress
McCas_H2_complete Thioridazine 0.1 mM
+0.2
+1.2
+0.4
N.D.
N.D.
no stress control
McCas_H2_complete no stress control
+0.2
+0.4
+1.3
N.D.
N.D.
stress
McCas_H2_complete sulfate 125 mM
+0.7
+0.3
+0.9
N.D.
N.D.
nutrient t2
H2 deepwell acetate and vitamin FREE
+0.6
+0.9
+0.4
N.D.
N.D.
stress
McCas_H2_complete Zinc Pyrithione 0.0142857142857143 mM
+0.8
+0.1
+1.1
N.D.
N.D.
no stress control
McCas_H2_complete no stress control
+0.6
+0.6
+0.7
N.D.
N.D.
stress
McCas_H2_complete potassium hexahydroxoantimonate 0.125 mM
+0.7
+0.6
+0.7
N.D.
N.D.
nitrogen source
H2 N-free with 10mM L-Glutamic
+0.2
+1.0
+0.8
N.D.
N.D.
stress
McCas_H2_complete Anhydrotetracycline 0.05 mM
+0.2
+0.9
+0.9
N.D.
N.D.
nutrient t2
H2 deepwell acetate FREE
+0.8
+0.3
+1.1
N.D.
N.D.
nitrogen source
H2 N-free with 10mM L-tyrosine
+0.8
+0.4
+1.1
N.D.
N.D.
stress
McCas_H2_complete potassium hexahydroxoantimonate 0.125 mM
+0.6
+1.3
+0.5
N.D.
N.D.
nitrogen source
H2 N-free with 10mM L-Glutamic
+0.5
+0.8
+1.1
N.D.
N.D.
rich t2
H2 McCas deepwell complete
+0.8
+0.4
+1.4
N.D.
N.D.
nitrogen source
H2 N-free with 5mM Inosine
+0.7
+0.4
+1.6
N.D.
N.D.
stress
McCas_H2_complete Spiramycin 0.1 mM
+0.1
+0.3
+2.3
N.D.
N.D.
nutrient t2
H2 deepwell vitamin FREE
+0.4
+0.3
+2.1
N.D.
N.D.
nitrogen source
H2 N-free with 10mM L-tyrosine
+0.9
+0.7
+2.0
N.D.
N.D.
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