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Fitness for 5 genes in
Methanococcus maripaludis S2
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500 nt
MMP_RS08295 and MMP_RS08300 are separated by 69 nucleotides
MMP_RS08300 and MMP_RS08305 are separated by 85 nucleotides
MMP_RS08305 and MMP_RS08310 are separated by 205 nucleotides
MMP_RS08310 and MMP_RS08315 are separated by 10 nucleotides
MMP_RS08295: MMP_RS08295 - histidine--tRNA ligase, at 1,559,794 to 1,561,044
_RS08295
MMP_RS08300: MMP_RS08300 - metal-sulfur cluster assembly factor, at 1,561,114 to 1,561,413
_RS08300
MMP_RS08305: MMP_RS08305 - aspartate--tRNA(Asn) ligase, at 1,561,499 to 1,562,815
_RS08305
MMP_RS08310: MMP_RS08310 - TMEM165/GDT1 family protein, at 1,563,021 to 1,563,284
_RS08310
MMP_RS08315: MMP_RS08315 - S-methyl-5-thioribose-1-phosphate isomerase, at 1,563,295 to 1,564,260
_RS08315
Group
Condition
MMP
_RS08295
MMP
_RS08300
MMP
_RS08305
MMP
_RS08310
MMP
_RS08315
stress
McCas_H2_complete Benzethonium chloride 0.003125 mM
N.D.
-6.6
N.D.
-0.1
+0.2
hydrogen no stress control
H2 no stress control
N.D.
-5.8
N.D.
+0.2
-0.4
stress
McCas_H2_complete Anhydrotetracycline 0.05 mM
N.D.
-6.2
N.D.
+0.2
+0.1
stress
McCas_H2_complete Rifampicin 0.5 mM
N.D.
-5.4
N.D.
+0.2
-0.6
stress
McCas_H2_complete dimetridazole 0.00833333333333333 mM
N.D.
-5.7
N.D.
+0.3
-0.3
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse2; 10 generations
N.D.
-5.8
N.D.
+0.3
+0.3
stress
McCas_H2_complete Anhydrotetracycline 0.05 mM
N.D.
-5.7
N.D.
+0.7
-0.0
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse3; 10 generations
N.D.
-5.7
N.D.
+0.7
+0.1
stress
McCas_H2_complete Spiramycin 0.1 mM
N.D.
-5.3
N.D.
+1.2
-0.6
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 10 generations
N.D.
-5.5
N.D.
+0.3
+0.6
stress
H2 McCas with 40 minute oxygen exposure
N.D.
-6.0
N.D.
+0.9
+0.5
stress
H2 McCas with 0.1 mM Na2S
N.D.
-5.9
N.D.
+1.0
+0.6
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations
N.D.
-5.5
N.D.
+1.0
+0.3
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse3; 20 generations
N.D.
-5.5
N.D.
+1.1
+0.2
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 10 generations
N.D.
-5.5
N.D.
+0.9
+0.4
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 10 generations
N.D.
-5.5
N.D.
+0.8
+0.5
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse2; 20 generations
N.D.
-5.4
N.D.
+1.0
+0.2
no stress control
McCas_H2_complete no stress control
N.D.
-5.8
N.D.
+1.1
+0.6
stress
McCas_H2_complete Sulfaquinoxaline 0.1 mM
N.D.
-6.3
N.D.
+1.9
+0.4
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations
N.D.
-5.6
N.D.
+1.0
+0.7
stress
McCas_H2_complete Calcium Chloride Dihydrate 83.3333333333333 mM
N.D.
-5.3
N.D.
+1.0
+0.5
no stress control
McCas_H2_complete no stress control
N.D.
-6.0
N.D.
+0.7
+1.5
nitrogen source
N2 fixation, H2 media
N.D.
-4.2
N.D.
+1.3
-0.8
stress
McCas_H2_complete Benzethonium chloride 0.003125 mM
N.D.
-5.4
N.D.
+1.3
+0.9
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
N.D.
-5.0
N.D.
+0.7
+1.3
stress
H2 McCas with 1 mM Na2S
N.D.
-4.7
N.D.
+1.0
+1.1
stress
H2 McCas with 1 hour oxygen exposure
N.D.
-4.6
N.D.
+1.3
+0.8
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations
N.D.
-4.5
N.D.
+0.8
+1.3
stress
McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM
N.D.
-5.2
N.D.
+1.6
+1.5
no stress control
McCas_H2_complete no stress control
N.D.
-3.9
N.D.
+2.4
+0.1
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