Fitness for 5 genes in Methanococcus maripaludis S2

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500 ntMMP_RS07755 and MMP_RS07760 are separated by 132 nucleotidesMMP_RS07760 and MMP_RS07765 are separated by 177 nucleotidesMMP_RS07765 and MMP_RS07770 are separated by 222 nucleotidesMMP_RS07770 and MMP_RS07775 are separated by 56 nucleotides MMP_RS07755: MMP_RS07755 - DUF555 domain-containing protein, at 1,466,991 to 1,467,332 _RS07755 MMP_RS07760: MMP_RS07760 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA, at 1,467,465 to 1,468,760 _RS07760 MMP_RS07765: MMP_RS07765 - sodium:alanine symporter family protein, at 1,468,938 to 1,470,299 _RS07765 MMP_RS07770: MMP_RS07770 - alanine racemase, at 1,470,522 to 1,471,643 _RS07770 MMP_RS07775: MMP_RS07775 - alanine dehydrogenase, at 1,471,700 to 1,472,821 _RS07775
Group Condition MMP_RS07755 MMP_RS07760 MMP_RS07765 MMP_RS07770 MMP_RS07775
nitrogen source H2 N-free with 10mM D-Alanine -1.6 N.D. -3.1 -3.4 -4.7
nitrogen source H2 N-free with 10mM D-Alanine -3.0 N.D. -2.5 -3.4 -3.8
nitrogen source H2 N-free with 10mM D-Alanine -1.7 N.D. -2.9 -3.3 -3.7
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -3.6 N.D. -4.0 -0.1 -2.8
stress McCas_formate_complete Calcium Chloride Dihydrate 166.666666666667 mM -4.1 N.D. -2.4 -0.7 -3.0
nitrogen source H2 N-free with 10mM L-Alanine -2.8 N.D. -2.7 -0.3 -3.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -2.4 N.D. -3.6 -0.1 -3.1
nitrogen source H2 N-free with 10mM L-Alanine -2.4 N.D. -2.5 -0.5 -3.5
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -5.1 N.D. -1.6 -1.0 -1.0
no stress control McCas_formate_complete no stress control -4.9 N.D. -1.7 +0.1 -1.5
stress McCas_formate_complete 1,2-dimethyl-5-nitroimidazole 0.00416666666666667 mM -4.5 N.D. -1.8 +0.1 -1.6
nitrogen source H2 N-free with 10mM L-Alanine -1.3 N.D. -2.4 -0.2 -3.7
stress McCas_formate_complete sulfate 125 mM -3.8 N.D. -1.9 +0.0 -1.8
stress McCas_formate_complete sulfate 125 mM -2.9 N.D. -2.5 -0.6 -1.3
stress McCas_formate_complete sodium bromide 250 mM -3.5 N.D. -2.1 -0.2 -1.3
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM -4.7 N.D. -1.5 +0.3 -0.3
stress McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM -5.4 N.D. +0.6 -0.2 -0.9
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations -5.6 N.D. +0.5 +0.2 +0.5
stress H2 McCas with 1 mM polysulfide -5.6 N.D. +0.6 +0.3 +0.4
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations -5.5 N.D. +0.6 +0.3 +0.5
stress H2 McCas with 10 mM polysulfide -5.6 N.D. +0.7 +0.4 +0.5
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse2; 20 generations -5.4 N.D. +0.7 +0.4 +0.6
stress McCas_formate_complete 3-nitroxypropanol 0.000416666666666667 mM -3.5 N.D. +2.5 +0.5 -1.2
stress McCas_formate_complete 3-nitroxypropanol 0.00833333333333333 mM -0.3 N.D. +10.1 +0.1 -1.4
nitrogen source H2 N-free with 10mM L-Threonine +2.0 N.D. +5.9 +0.9 +0.2
nitrogen source H2 N-free with 10mM L-Threonine +1.8 N.D. +7.1 +0.5 +0.6
nitrogen source H2 N-free with 10mM L-Serine -0.3 N.D. +13.0 +0.2 +0.2
nitrogen source H2 N-free with 10mM B-Alanine +0.2 N.D. +13.3 -0.3 +0.2
nitrogen source H2 N-free with 10mM L-Serine +0.1 N.D. +13.7 -0.4 -0.0
nitrogen source H2 N-free with 10mM B-Alanine -0.3 N.D. +13.3 +0.5 -0.1
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