Fitness for 5 genes in Methanococcus maripaludis S2

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500 ntMMP_RS07555 and MMP_RS07560 are separated by 173 nucleotidesMMP_RS07560 and MMP_RS07565 are separated by 129 nucleotidesMMP_RS07565 and MMP_RS07570 are separated by 101 nucleotidesMMP_RS07570 and MMP_RS07575 are separated by 15 nucleotides MMP_RS07555: MMP_RS07555 - monovalent cation/H+ antiporter subunit E, at 1,429,995 to 1,430,315 _RS07555 MMP_RS07560: MMP_RS07560 - prefoldin subunit alpha, at 1,430,489 to 1,430,923 _RS07560 MMP_RS07565: MMP_RS07565 - tRNA-Val, at 1,431,053 to 1,431,129 _RS07565 MMP_RS07570: MMP_RS07570 - nucleotidyltransferase family protein, at 1,431,231 to 1,432,280 _RS07570 MMP_RS07575: MMP_RS07575 - RNA ligase partner protein, at 1,432,296 to 1,432,967 _RS07575
Group Condition MMP_RS07555 MMP_RS07560 MMP_RS07565 MMP_RS07570 MMP_RS07575
nutrient t2 Formate deepwell acetate and casamino FREE -5.2 +0.4 N.D. +0.0 -4.3
nutrient t2 H2 deepwell acetate FREE -4.8 +1.0 N.D. +0.5 -5.8
nutrient t2 H2 deepwell casamino FREE -5.0 +0.2 N.D. +0.1 -3.8
nutrient t2 H2 deepwell acetate and vitamin FREE -4.8 -0.0 N.D. +0.2 -3.8
nutrient t1 Formate deepwell casamino FREE -4.2 +0.1 N.D. -0.0 -4.2
nutrient t2 H2 deepwell vitamin and casamino FREE -4.9 +0.3 N.D. +0.1 -3.6
nutrient t2 H2 deepwell vitamin and casamino FREE -4.7 +0.5 N.D. +0.4 -4.0
nutrient t2 H2 deepwell casamino FREE -4.9 +0.2 N.D. +0.4 -3.3
stress McCas_H2_complete Nitrofurantoin 0.1 mM -5.8 -0.1 N.D. -0.1 -1.7
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations -5.1 -0.8 N.D. -0.5 -1.2
stress Formate 100 mM hypophosphite -4.9 +0.1 N.D. +0.2 -2.9
nutrient t2 H2 deepwell acetate and casamino FREE -5.1 +0.3 N.D. +0.2 -2.9
nutrient t2 Formate deepwell autotrophic conditions -3.8 +0.4 N.D. +0.2 -4.2
nutrient t2 H2 deepwell vitamin and casamino FREE -4.6 +0.5 N.D. +0.3 -3.6
nutrient t2 H2 deepwell acetate FREE -3.7 +1.0 N.D. -0.3 -4.3
nutrient t2 H2 deepwell acetate FREE -4.7 +0.4 N.D. -0.4 -2.6
nutrient t1 Formate deepwell casamino FREE -3.5 +0.4 N.D. -0.0 -4.1
stress Formate 100 mM hypophosphite -4.1 -0.1 N.D. +0.4 -3.1
no stress control McCas_H2_complete no stress control -5.7 +0.5 N.D. -0.2 -1.4
nutrient t2 H2 deepwell acetate and casamino FREE -4.8 +0.8 N.D. +0.4 -3.2
nutrient t2 Formate deepwell acetate and casamino FREE -2.3 +0.4 N.D. -0.1 -4.7
nutrient t2 Formate deepwell acetate and casamino FREE -2.8 +0.4 N.D. -0.1 -4.0
stress Formate 100 mM hypophosphite -4.6 +0.1 N.D. +0.3 -2.3
nitrogen source H2 N-free with 10mM L-Isoleucine -4.5 +0.5 N.D. -0.1 -2.2
nutrient t2 H2 deepwell autotrophic conditions -3.6 +0.3 N.D. +0.4 -3.2
nutrient t2 H2 deepwell autotrophic conditions -3.1 +0.3 N.D. +0.6 -3.4
natural competence H2 McCas- cells grown in liquid medium with neomycin +6.1 +0.5 N.D. +0.2 -0.8
natural competence H2 McCas- cells grown in liquid medium with neomycin +7.7 +0.2 N.D. +0.2 +0.1
stress Formate with Neomycin 12.5 mM +9.1 +0.0 N.D. -0.4 -0.2
natural competence H2 McCas- cells grown in liquid medium with neomycin +8.2 +0.4 N.D. +0.1 +0.1
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