Fitness for 5 genes in Methanococcus maripaludis S2

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500 ntMMP_RS07335 and MMP_RS07340 are separated by 23 nucleotidesMMP_RS07340 and MMP_RS07345 are separated by 3 nucleotidesMMP_RS07345 and MMP_RS07350 are separated by 72 nucleotidesMMP_RS07350 and MMP_RS07355 are separated by 54 nucleotides MMP_RS07335: MMP_RS07335 - tRNA (cytidine(56)-2'-O)-methyltransferase, at 1,398,277 to 1,398,816 _RS07335 MMP_RS07340: MMP_RS07340 - dCTP deaminase, at 1,398,840 to 1,399,460 _RS07340 MMP_RS07345: MMP_RS07345 - glycine synthesis protein GlyXL (from data), at 1,399,464 to 1,400,840 _RS07345 MMP_RS07350: MMP_RS07350 - preprotein translocase subunit Sec61beta, at 1,400,913 to 1,401,074 _RS07350 MMP_RS07355: MMP_RS07355 - transcription factor S, at 1,401,129 to 1,401,446 _RS07355
Group Condition MMP_RS07335 MMP_RS07340 MMP_RS07345 MMP_RS07350 MMP_RS07355
nutrient t2 H2 deepwell casamino FREE -0.4 N.D. -7.4 -0.3 -5.9
nutrient t2 H2 deepwell casamino FREE -0.5 N.D. -6.9 -3.8 -2.4
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations +0.3 N.D. +0.9 -7.4 -6.3
nutrient t2 H2 deepwell vitamin and casamino FREE -0.1 N.D. -6.9 +0.3 -5.5
nutrient t2 H2 deepwell acetate and casamino FREE -0.7 N.D. -5.7 -0.8 -4.9
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.2 N.D. -7.5 -1.5 -2.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.0 N.D. -7.1 -0.7 -3.5
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +0.0 N.D. -7.5 -0.7 -2.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE -0.1 N.D. -7.4 -1.9 -1.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE -0.2 N.D. -7.2 -1.0 -2.1
nutrient t2 H2 deepwell autotrophic conditions -0.5 N.D. -6.4 -0.2 -3.2
nutrient t2 H2 deepwell vitamin and casamino FREE -0.5 N.D. -6.8 -0.9 -2.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE -0.0 N.D. -7.3 -0.8 -2.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.0 N.D. -7.3 -0.8 -1.7
nutrient t2 Formate deepwell acetate and casamino FREE -0.1 N.D. -7.4 -0.3 -2.0
nutrient t2 H2 deepwell autotrophic conditions -0.1 N.D. -6.5 -1.3 -1.8
nutrient t2 H2 deepwell acetate and casamino FREE -0.0 N.D. -6.1 -1.5 -2.1
nitrogen source H2 N-free with 10mM L-Arginine +0.1 N.D. -6.5 -0.1 -3.1
nitrogen source H2 N-free with 2.5mM Cytosine +0.1 N.D. -4.9 +0.9 -5.3
nitrogen source H2 N-free with 10mM L-Lysine +0.3 N.D. -4.7 +0.4 -5.0
nitrogen source H2 N-free with 10mM L-Leucine -0.0 N.D. -5.8 +0.7 -3.8
nitrogen source H2 N-free with 10mM D-glucosamine +0.3 N.D. -5.6 +0.6 -3.8
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse2; 20 generations +0.3 N.D. +1.2 -6.1 -3.3
nitrogen source H2 N-free with 10mM L-Methionine +0.1 N.D. -5.0 +0.7 -3.6
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations +0.4 N.D. +1.2 -8.3 +0.1
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 20 generations +0.5 N.D. +0.9 -8.3 +1.5
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations -0.2 N.D. +0.7 -7.9 +2.5
nutrient t1 Mc formate, casamino free, with 10mM L-Threonine -2.8 N.D. -3.7 +0.0 +3.4
nutrient t1 Mc formate, casamino free, with 10mM L-Threonine -2.6 N.D. -3.6 +0.5 +4.5
nutrient t1 Mc formate, casamino free, with 10mM L-Threonine -2.2 N.D. -4.0 +0.1 +5.2
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