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Fitness for 5 genes in
Methanococcus maripaludis S2
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500 nt
MMP_RS06740 and MMP_RS06745 are separated by 75 nucleotides
MMP_RS06745 and MMP_RS06750 are separated by 35 nucleotides
MMP_RS06750 and MMP_RS06755 are separated by 29 nucleotides
MMP_RS06755 and MMP_RS06760 are separated by 17 nucleotides
MMP_RS06740: MMP_RS06740 - fructose-6-phosphate aldolase, at 1,294,684 to 1,295,331
_RS06740
MMP_RS06745: MMP_RS06745 - DUF2119 domain-containing protein, at 1,295,407 to 1,296,018
_RS06745
MMP_RS06750: MMP_RS06750 - IMP cyclohydrolase, at 1,296,054 to 1,296,659
_RS06750
MMP_RS06755: MMP_RS06755 - 4Fe-4S binding protein, at 1,296,689 to 1,296,847
_RS06755
MMP_RS06760: MMP_RS06760 - hypothetical protein, at 1,296,865 to 1,297,491
_RS06760
Group
Condition
MMP
_RS06740
MMP
_RS06745
MMP
_RS06750
MMP
_RS06755
MMP
_RS06760
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations
N.D.
+0.0
N.D.
N.D.
-4.6
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
N.D.
-0.4
N.D.
N.D.
-3.3
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 20 generations
N.D.
-0.0
N.D.
N.D.
-3.0
stress
Formate McCas no stress control 2
N.D.
-0.7
N.D.
N.D.
-2.1
nutrient t2
Formate deepwell acetate FREE
N.D.
-0.8
N.D.
N.D.
-1.6
stress
H2 50 mM hypophosphite
N.D.
+0.1
N.D.
N.D.
-2.1
stress
Formate with Bacitracin 15.6 uM
N.D.
-0.8
N.D.
N.D.
-1.2
stress
McCas_H2_complete 2-bromoethanesulfonic 0.00833333333333333 mM
N.D.
-1.1
N.D.
N.D.
-0.9
stress
McCas_H2_complete 5-Fluorouracil 0.00833333333333333 mM
N.D.
-0.9
N.D.
N.D.
-1.0
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 10 generations
N.D.
-0.4
N.D.
N.D.
-1.5
stress
McCas_formate_complete Rifampicin 0.5 mM
N.D.
+0.3
N.D.
N.D.
-1.9
stress
McCas_H2_complete Benzethonium chloride 0.00625 mM
N.D.
+0.2
N.D.
N.D.
-1.6
stress
Formate with Bacitracin 15.6 uM
N.D.
+0.2
N.D.
N.D.
-1.4
stress
McCas_formate_complete Fluorophosphate 62.5 mM
N.D.
+0.2
N.D.
N.D.
-1.4
stress
McCas_H2_complete 2-Mercaptopyridine N-oxide 0.02 mM
N.D.
+0.3
N.D.
N.D.
-1.3
stress
McCas_H2_complete Benzethonium chloride 0.003125 mM
N.D.
+0.6
N.D.
N.D.
-1.3
stress
McCas_formate_complete Polymyxin B sulfate 0.1 mM
N.D.
+0.5
N.D.
N.D.
-1.2
stress
McCas_formate_complete Econazole nitrate salt 0.02 mM
N.D.
+0.5
N.D.
N.D.
-1.1
stress
McCas_H2_complete Thioridazine 0.1 mM
N.D.
+0.6
N.D.
N.D.
-0.9
stress
McCas_H2_complete Rifampicin 0.5 mM
N.D.
+1.2
N.D.
N.D.
-1.2
stress
McCas_formate_complete Rifampicin 0.5 mM
N.D.
+1.2
N.D.
N.D.
-1.1
stress
McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM
N.D.
+0.9
N.D.
N.D.
-0.8
stress
McCas_formate_complete Phenazine methosulfate 0.0333333333333333 mM
N.D.
+1.2
N.D.
N.D.
-0.9
nutrient t1
Mc formate, casamino free, with 10mM L-Threonine
N.D.
+1.0
N.D.
N.D.
-0.5
nutrient t2
H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
N.D.
-0.7
N.D.
N.D.
+1.5
stress
McCas_H2_complete 1,2-dimethyl-5-nitroimidazole 0.00833333333333333 mM
N.D.
-0.4
N.D.
N.D.
+1.1
no stress control
McCas_H2_complete no stress control
N.D.
+1.3
N.D.
N.D.
-0.3
stress
McCas_H2_complete Chloride 250 mM
N.D.
-0.1
N.D.
N.D.
+1.5
nutrient t2
H2 deepwell acetate and casamino FREE
N.D.
+0.9
N.D.
N.D.
+0.6
nutrient t2
H2 deepwell autotrophic conditions
N.D.
+0.9
N.D.
N.D.
+0.7
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