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Fitness for 5 genes in
Methanococcus maripaludis S2
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500 nt
MMP_RS06235 and MMP_RS06240 are separated by 18 nucleotides
MMP_RS06240 and MMP_RS06245 are separated by 38 nucleotides
MMP_RS06245 and MMP_RS06250 are separated by 41 nucleotides
MMP_RS06250 and MMP_RS06255 are separated by 98 nucleotides
MMP_RS06235: MMP_RS06235 - hydroxymethylglutaryl-CoA synthase, at 1,198,055 to 1,199,104
_RS06235
MMP_RS06240: MMP_RS06240 - thiolase domain-containing protein, at 1,199,123 to 1,200,301
_RS06240
MMP_RS06245: MMP_RS06245 - Zn-ribbon domain-containing OB-fold protein, at 1,200,340 to 1,200,732
_RS06245
MMP_RS06250: MMP_RS06250 - hypothetical protein, at 1,200,774 to 1,201,451
_RS06250
MMP_RS06255: MMP_RS06255 - cobyric acid synthase CobQ, at 1,201,550 to 1,203,028
_RS06255
Group
Condition
MMP
_RS06235
MMP
_RS06240
MMP
_RS06245
MMP
_RS06250
MMP
_RS06255
stress
McCas_formate_complete Polymyxin B sulfate 0.1 mM
N.D.
N.D.
N.D.
-0.6
N.D.
stress
McCas_H2_complete Bacitracin 0.003125 mM
N.D.
N.D.
N.D.
-0.6
N.D.
nitrogen source
Formate N-free with 2mM Adenosine
N.D.
N.D.
N.D.
-0.5
N.D.
stress
McCas_H2_complete potassium hexahydroxoantimonate 0.125 mM
N.D.
N.D.
N.D.
-0.5
N.D.
stress
McCas_H2_complete Chloride 250 mM
N.D.
N.D.
N.D.
+0.5
N.D.
nutrient t2
H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
N.D.
N.D.
N.D.
+0.5
N.D.
stress
McCas_H2_complete metronidazole 0.000833333333333333
N.D.
N.D.
N.D.
+0.5
N.D.
stress
H2 McCas with 1 hour oxygen exposure
N.D.
N.D.
N.D.
+0.5
N.D.
no stress control
McCas_formate_complete no stress control
N.D.
N.D.
N.D.
+0.5
N.D.
stress
McCas_formate_complete 3-nitroxypropanol 0.0015625 mM
N.D.
N.D.
N.D.
+0.5
N.D.
stress
McCas_formate_complete 3-nitroxypropanol 0.00833333333333333 mM
N.D.
N.D.
N.D.
+0.5
N.D.
stress
McCas_formate_complete 2-bromoethanesulfonic 4.16666666666667E-05 mM
N.D.
N.D.
N.D.
+0.5
N.D.
stress
McCas_formate_complete gold (III) chloride 0.0714285714285714 mM
N.D.
N.D.
N.D.
+0.5
N.D.
nutrient t1
H2 deepwell vitamin FREE
N.D.
N.D.
N.D.
+0.5
N.D.
nitrogen source
H2 N-free with 10mM L-Phenylalanine
N.D.
N.D.
N.D.
+0.5
N.D.
nitrogen source
H2 N-free with 10mM Cytidine
N.D.
N.D.
N.D.
+0.5
N.D.
nitrogen source
H2 N-free with 2mM Thymine
N.D.
N.D.
N.D.
+0.5
N.D.
stress
McCas_formate_complete dimetridazole 0.00416666666666667 mM
N.D.
N.D.
N.D.
+0.5
N.D.
stress
McCas_formate_complete Calcium Chloride Dihydrate 166.666666666667 mM
N.D.
N.D.
N.D.
+0.5
N.D.
stress
H2 McCas with 40 minute oxygen exposure
N.D.
N.D.
N.D.
+0.5
N.D.
nitrogen source
H2 N-free with 10mM L-Phenylalanine
N.D.
N.D.
N.D.
+0.6
N.D.
nutrient t1
Mc formate, casamino free, with 10mM L-Methionine
N.D.
N.D.
N.D.
+0.6
N.D.
nitrogen source
H2 N-free with 10mM L-Proline
N.D.
N.D.
N.D.
+0.6
N.D.
nitrogen source
N2 fixation, H2 media
N.D.
N.D.
N.D.
+0.6
N.D.
stress
McCas_H2_complete dimetridazole 0.00833333333333333 mM
N.D.
N.D.
N.D.
+0.7
N.D.
stress
McCas_H2_complete potassium hexahydroxoantimonate 0.125 mM
N.D.
N.D.
N.D.
+0.7
N.D.
stress
McCas_H2_complete Cesium chloride 41.6666666666667 mM
N.D.
N.D.
N.D.
+0.8
N.D.
stress
McCas_H2_complete Econazole nitrate salt 0.02 mM
N.D.
N.D.
N.D.
+0.8
N.D.
stress
McCas_formate_complete Antimycin A 0.01 mM
N.D.
N.D.
N.D.
+0.9
N.D.
stress
McCas_H2_complete Bacitracin 0.003125 mM
N.D.
N.D.
N.D.
+0.9
N.D.
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