Fitness for 5 genes in Methanococcus maripaludis S2

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500 ntMMP_RS02320 and MMP_RS02325 overlap by 4 nucleotidesMMP_RS02325 and MMP_RS02330 are separated by 93 nucleotidesMMP_RS02330 and MMP_RS02335 are separated by 155 nucleotidesMMP_RS02335 and MMP_RS02340 are separated by 47 nucleotides MMP_RS02320: MMP_RS02320 - glutamate-cysteine ligase family protein, at 436,573 to 437,676 _RS02320 MMP_RS02325: MMP_RS02325 - epoxyqueuosine reductase, at 437,673 to 438,470 _RS02325 MMP_RS02330: MMP_RS02330 - dihydroorotate dehydrogenase, at 438,564 to 439,478 _RS02330 MMP_RS02335: MMP_RS02335 - DNA-directed RNA polymerase, at 439,634 to 440,197 _RS02335 MMP_RS02340: MMP_RS02340 - DNA-directed RNA polymerase subunit E'', at 440,245 to 440,421 _RS02340
Group Condition MMP_RS02320 MMP_RS02325 MMP_RS02330 MMP_RS02335 MMP_RS02340
nitrogen source H2 N-free with 10mM Cytidine +0.2 +0.2 N.D. N.D. -6.4
nutrient t1 Mc formate, casamino free, with 10mM L-Methionine -0.2 -0.0 N.D. N.D. -4.8
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +0.1 +0.1 N.D. N.D. -5.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.5 -0.0 N.D. N.D. -5.4
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.1 +0.1 N.D. N.D. -4.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.4 -0.1 N.D. N.D. -4.8
nitrogen source H2 N-free with 10mM L-Methionine -0.0 -0.1 N.D. N.D. -4.3
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations +0.1 +0.2 N.D. N.D. -4.5
nutrient t2 Formate deepwell autotrophic conditions +0.1 -0.0 N.D. N.D. -4.3
nutrient t2 H2 deepwell vitamin and casamino FREE -0.2 -0.2 N.D. N.D. -3.8
stress Formate 100 mM hypophosphite +0.1 -0.0 N.D. N.D. -4.1
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.2 -0.1 N.D. N.D. -4.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.4 -0.0 N.D. N.D. -4.4
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse2; 20 generations +0.2 -0.0 N.D. N.D. -4.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +0.2 +0.2 N.D. N.D. -4.2
nutrient t1 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.2 -0.0 N.D. N.D. -4.0
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations +0.3 -0.1 N.D. N.D. -3.9
nutrient t2 Formate deepwell acetate and casamino FREE +0.1 -0.1 N.D. N.D. -3.7
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.0 +0.1 N.D. N.D. -3.7
nutrient t2 Formate deepwell vitamin and casamino FREE +0.2 -0.1 N.D. N.D. -3.6
stress McCas_H2_complete Calcium Chloride Dihydrate 83.3333333333333 mM +0.2 +0.3 N.D. N.D. -4.0
nutrient t2 Formate deepwell autotrophic conditions +0.1 +0.0 N.D. N.D. -3.6
nitrogen source N2 fixation, H2 media +0.2 -0.2 N.D. N.D. -3.5
nutrient t2 Formate deepwell acetate FREE +0.1 -0.4 N.D. N.D. -3.1
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse3; 20 generations +0.1 +0.1 N.D. N.D. -3.6
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM +0.0 +0.2 N.D. N.D. -3.5
nitrogen source H2 N-free with 2.5mM Cytosine +0.3 +0.1 N.D. N.D. -3.7
nitrogen source H2 N-free with 10mM L-Glutamic +0.6 +0.5 N.D. N.D. -2.7
nitrogen source H2 N-free with 10mM Uridine +1.3 +0.8 N.D. N.D. -2.9
stress McCas_H2_complete sodium iodide 250 mM +0.0 +0.2 N.D. N.D. +3.6
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