Fitness for 5 genes in Methanococcus maripaludis S2

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500 ntMMP_RS01520 and MMP_RS01525 are separated by 188 nucleotidesMMP_RS01525 and MMP_RS01530 are separated by 150 nucleotidesMMP_RS01530 and MMP_RS01535 are separated by 134 nucleotidesMMP_RS01535 and MMP_RS01540 are separated by 9 nucleotides MMP_RS01520: MMP_RS01520 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 283,401 to 283,877 _RS01520 MMP_RS01525: MMP_RS01525 - nucleoside-diphosphate kinase, at 284,066 to 284,485 _RS01525 MMP_RS01530: MMP_RS01530 - translation initiation factor IF-2, at 284,636 to 286,441 _RS01530 MMP_RS01535: MMP_RS01535 - DHH family phosphoesterase, at 286,576 to 288,036 _RS01535 MMP_RS01540: MMP_RS01540 - DUF504 domain-containing protein, at 288,046 to 288,276 _RS01540
Group Condition MMP_RS01520 MMP_RS01525 MMP_RS01530 MMP_RS01535 MMP_RS01540
nutrient t2 H2 deepwell acetate and casamino FREE N.D. -2.5 N.D. N.D. -3.3
stress Formate McCas no stress control 2 N.D. -2.7 N.D. N.D. -0.6
nutrient t2 H2 deepwell autotrophic conditions N.D. +0.0 N.D. N.D. -2.6
nutrient t2 H2 deepwell acetate and casamino FREE N.D. -0.5 N.D. N.D. -2.0
nutrient t2 H2 deepwell acetate and casamino FREE N.D. -0.6 N.D. N.D. -1.8
nutrient t2 Formate deepwell acetate FREE N.D. -1.8 N.D. N.D. -0.5
nutrient t2 H2 deepwell acetate and casamino FREE N.D. +0.0 N.D. N.D. -2.4
stress McCas_H2_complete Lithium chloride 250 mM N.D. -0.8 N.D. N.D. -1.4
stress McCas_H2_complete 2-bromoethanesulfonic 0.00833333333333333 mM N.D. +0.7 N.D. N.D. -2.4
natural competence H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin N.D. -2.0 N.D. N.D. +0.3
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM N.D. +0.7 N.D. N.D. -2.0
nitrogen source H2 N-free with 10mM L-Phenylalanine N.D. +0.7 N.D. N.D. -1.4
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations N.D. +2.0 N.D. N.D. -2.7
nutrient t2 H2 deepwell acetate and vitamin FREE N.D. +1.2 N.D. N.D. -1.6
stress McCas_formate_complete 2-bromoethanesulfonic 4.16666666666667E-05 mM N.D. +1.8 N.D. N.D. -1.1
stress McCas_H2_complete metronidazole 0.000833333333333333 N.D. +1.5 N.D. N.D. -0.7
no stress control McCas_H2_complete no stress control N.D. +1.6 N.D. N.D. -0.8
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations N.D. +2.0 N.D. N.D. -0.2
stress McCas_H2_complete dimetridazole 0.00833333333333333 mM N.D. +2.3 N.D. N.D. -0.4
no stress control McCas_H2_complete no stress control N.D. +1.1 N.D. N.D. +1.1
stress McCas_formate_complete Thioridazine 0.05 mM N.D. +0.7 N.D. N.D. +1.4
nutrient t1 Mc formate, casamino free, with 10mM L-Threonine N.D. +1.1 N.D. N.D. +1.2
nutrient t2 H2 deepwell acetate FREE N.D. +1.1 N.D. N.D. +1.3
stress McCas_formate_complete Phenazine methosulfate 0.0333333333333333 mM N.D. +1.0 N.D. N.D. +1.5
stress McCas_formate_complete 3-nitroxypropanol 0.000416666666666667 mM N.D. +1.1 N.D. N.D. +1.5
stress McCas_formate_complete nitrate 35.7142857142857 mM N.D. +1.6 N.D. N.D. +1.2
stress McCas_H2_complete Thioridazine 0.1 mM N.D. +3.1 N.D. N.D. -0.2
stress McCas_formate_complete gold (III) chloride 0.0714285714285714 mM N.D. +1.3 N.D. N.D. +1.8
stress McCas_H2_complete dimetridazole 0.000833333333333333 mM N.D. +2.3 N.D. N.D. +0.9
stress McCas_H2_complete metavanadate 4.54545454545455 mM N.D. +1.3 N.D. N.D. +2.9
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