Fitness for 5 genes in Methanococcus maripaludis S2

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500 ntMMP_RS01505 and MMP_RS01510 are separated by 20 nucleotidesMMP_RS01510 and MMP_RS01515 are separated by 60 nucleotidesMMP_RS01515 and MMP_RS01520 are separated by 1 nucleotidesMMP_RS01520 and MMP_RS01525 are separated by 188 nucleotides MMP_RS01505: MMP_RS01505 - hypothetical protein, at 280,059 to 281,036 _RS01505 MMP_RS01510: MMP_RS01510 - phosphoribosyl-AMP cyclohydrolase, at 281,057 to 281,446 _RS01510 MMP_RS01515: MMP_RS01515 - PINc/VapC family ATPase, at 281,507 to 283,399 _RS01515 MMP_RS01520: MMP_RS01520 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 283,401 to 283,877 _RS01520 MMP_RS01525: MMP_RS01525 - nucleoside-diphosphate kinase, at 284,066 to 284,485 _RS01525
Group Condition MMP_RS01505 MMP_RS01510 MMP_RS01515 MMP_RS01520 MMP_RS01525
stress Formate McCas no stress control 2 N.D. N.D. -0.0 N.D. -2.7
nutrient t2 H2 deepwell acetate and casamino FREE N.D. N.D. +0.3 N.D. -2.5
natural competence H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin N.D. N.D. -0.0 N.D. -2.0
nutrient t2 Formate deepwell acetate FREE N.D. N.D. +0.1 N.D. -1.8
nitrogen source H2 N-free with 10mM Uridine N.D. N.D. -0.4 N.D. -1.1
stress McCas_H2_complete Benzethonium chloride 0.003125 mM N.D. N.D. +0.2 N.D. -1.6
stress McCas_H2_complete Lithium chloride 250 mM N.D. N.D. -0.8 N.D. +0.8
stress McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM N.D. N.D. +0.8 N.D. -0.8
nutrient t1 Mc formate, casamino free, with 10mM L-Threonine N.D. N.D. -0.4 N.D. +1.1
nitrogen source H2 N-free with 10mM Gly-Glu N.D. N.D. -0.4 N.D. +1.3
stress McCas_H2_complete metavanadate 4.54545454545455 mM N.D. N.D. -0.3 N.D. +1.3
stress McCas_H2_complete Antimycin A 0.01 mM N.D. N.D. +0.7 N.D. +0.8
stress McCas_H2_complete sulfate 125 mM N.D. N.D. +1.0 N.D. +0.6
no stress control McCas_H2_complete no stress control N.D. N.D. -0.1 N.D. +1.6
stress McCas_H2_complete Anhydrotetracycline 0.05 mM N.D. N.D. +0.5 N.D. +1.0
no stress control McCas_H2_complete no stress control N.D. N.D. +0.3 N.D. +1.3
stress McCas_H2_complete Antimycin A 0.01 mM N.D. N.D. +0.6 N.D. +1.0
stress McCas_H2_complete Chloride 500 mM N.D. N.D. +0.9 N.D. +0.8
stress McCas_H2_complete metronidazole 0.000833333333333333 N.D. N.D. +0.1 N.D. +1.5
stress McCas_formate_complete nitrate 35.7142857142857 mM N.D. N.D. +0.1 N.D. +1.6
nutrient t2 H2 deepwell acetate and vitamin FREE N.D. N.D. +0.4 N.D. +1.3
stress McCas_H2_complete Chloride 250 mM N.D. N.D. +0.7 N.D. +1.0
stress McCas_H2_complete metronidazole 0.00833333333333333 mM N.D. N.D. +0.3 N.D. +1.6
stress McCas_formate_complete 2-bromoethanesulfonic 4.16666666666667E-05 mM N.D. N.D. +0.1 N.D. +1.8
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations N.D. N.D. +0.1 N.D. +2.0
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations N.D. N.D. +0.3 N.D. +2.0
stress McCas_H2_complete Fluorophosphate 62.5 mM N.D. N.D. +1.2 N.D. +1.2
stress McCas_H2_complete dimetridazole 0.000833333333333333 mM N.D. N.D. +0.4 N.D. +2.3
stress McCas_H2_complete dimetridazole 0.00833333333333333 mM N.D. N.D. +0.5 N.D. +2.3
stress McCas_H2_complete Thioridazine 0.1 mM N.D. N.D. -0.1 N.D. +3.1
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