Fitness for 5 genes in Methanococcus maripaludis S2

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500 ntMMP_RS01030 and MMP_RS01035 are separated by 14 nucleotidesMMP_RS01035 and MMP_RS01040 are separated by 69 nucleotidesMMP_RS01040 and MMP_RS01045 are separated by 89 nucleotidesMMP_RS01045 and MMP_RS01050 are separated by 84 nucleotides MMP_RS01030: MMP_RS01030 - MTAP family purine nucleoside phosphorylase, at 193,763 to 194,524 _RS01030 MMP_RS01035: MMP_RS01035 - threonylcarbamoyl-AMP synthase, at 194,539 to 195,159 _RS01035 MMP_RS01040: MMP_RS01040 - phosphomethylpyrimidine synthase, at 195,229 to 196,509 _RS01040 MMP_RS01045: MMP_RS01045 - diphthamide biosynthesis enzyme Dph2, at 196,599 to 197,528 _RS01045 MMP_RS01050: MMP_RS01050 - tRNA-Leu, at 197,613 to 197,697 _RS01050
Group Condition MMP_RS01030 MMP_RS01035 MMP_RS01040 MMP_RS01045 MMP_RS01050
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -2.3 N.D. N.D. -2.1 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -2.1 N.D. N.D. -1.9 N.D.
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse2; 20 generations -1.1 N.D. N.D. -2.9 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -1.9 N.D. N.D. -1.9 N.D.
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse3; 20 generations -1.1 N.D. N.D. -2.6 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -2.2 N.D. N.D. -1.5 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -2.0 N.D. N.D. -1.7 N.D.
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse3; 20 generations -1.0 N.D. N.D. -2.5 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -1.9 N.D. N.D. -1.6 N.D.
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations -1.1 N.D. N.D. -2.4 N.D.
stress Formate 100 mM hypophosphite -1.3 N.D. N.D. -2.2 N.D.
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations -0.4 N.D. N.D. -3.0 N.D.
nutrient t2 H2 deepwell vitamin FREE -1.0 N.D. N.D. -2.3 N.D.
stress H2 McCas with 1 mM Na2S -1.3 N.D. N.D. -2.0 N.D.
nutrient t2 H2 deepwell acetate FREE -1.4 N.D. N.D. -1.9 N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -1.5 N.D. N.D. -1.7 N.D.
nutrient t2 H2 deepwell acetate FREE -1.6 N.D. N.D. -1.5 N.D.
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations -1.4 N.D. N.D. -1.7 N.D.
rich t2 H2 McCas deepwell complete -1.5 N.D. N.D. -1.5 N.D.
stress H2 McCas with 1 mM polysulfide -1.6 N.D. N.D. -1.5 N.D.
stress Formate 100 mM hypophosphite -0.9 N.D. N.D. -2.1 N.D.
stress H2 McCas with 10 mM Na2S -1.5 N.D. N.D. -1.5 N.D.
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations -0.7 N.D. N.D. -2.3 N.D.
nutrient t2 H2 deepwell acetate and vitamin FREE -1.0 N.D. N.D. -2.0 N.D.
stress H2 McCas with 10 mM (NH4)2S -1.5 N.D. N.D. -1.4 N.D.
mixed community Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse2; 20 generations -0.5 N.D. N.D. -2.4 N.D.
stress H2 McCas with 1 mM (NH4)2S control -1.4 N.D. N.D. -1.5 N.D.
nutrient t2 H2 deepwell acetate and vitamin FREE -1.4 N.D. N.D. -1.5 N.D.
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -0.6 N.D. N.D. -2.2 N.D.
nitrogen source H2 N-free with 10mM Uridine +2.9 N.D. N.D. -0.0 N.D.
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