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Fitness for 5 genes in
Methanococcus maripaludis S2
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500 nt
MMP_RS00060 and MMP_RS00065 are separated by 381 nucleotides
MMP_RS00065 and MMP_RS00070 are separated by 28 nucleotides
MMP_RS00070 and MMP_RS00075 are separated by 10 nucleotides
MMP_RS00075 and MMP_RS00080 are separated by 26 nucleotides
MMP_RS00060: MMP_RS00060 - 3-dehydroquinate synthase II, at 15,336 to 16,421
_RS00060
MMP_RS00065: MMP_RS00065 - geranylgeranylglyceryl/heptaprenylglyceryl phosphate synthase, at 16,803 to 17,573
_RS00065
MMP_RS00070: MMP_RS00070 - DNA-directed DNA polymerase II small subunit, at 17,602 to 19,359
_RS00070
MMP_RS00075: MMP_RS00075 - hypothetical protein, at 19,370 to 20,479
_RS00075
MMP_RS00080: MMP_RS00080 - RecB-family nuclease, at 20,506 to 20,931
_RS00080
Group
Condition
MMP
_RS00060
MMP
_RS00065
MMP
_RS00070
MMP
_RS00075
MMP
_RS00080
stress
McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM
-5.1
N.D.
N.D.
N.D.
+0.5
nitrogen source
H2 N-free with 5mM Inosine
-4.2
N.D.
N.D.
N.D.
-0.0
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations
-3.0
N.D.
N.D.
N.D.
-0.0
nutrient t2
H2 deepwell acetate and casamino FREE
-1.5
N.D.
N.D.
N.D.
-0.8
stress
H2 McCas with 10 mM (NH4)2S
-1.7
N.D.
N.D.
N.D.
-0.5
stress
H2 McCas with 0.1 mM (NH4)2S
-2.3
N.D.
N.D.
N.D.
+0.2
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 10 generations
-2.0
N.D.
N.D.
N.D.
-0.1
nitrogen source
H2 N-free with 10mM Glycine
-1.7
N.D.
N.D.
N.D.
-0.4
iron
Iron excess (5x) , H2 media
-1.8
N.D.
N.D.
N.D.
-0.2
nutrient t2
Formate deepwell acetate FREE
-2.5
N.D.
N.D.
N.D.
+0.5
nutrient t2
H2 deepwell autotrophic conditions
-1.3
N.D.
N.D.
N.D.
-0.7
mixed community
Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 20 generations
-2.1
N.D.
N.D.
N.D.
+0.1
rich t2
H2 McCas deepwell complete
-1.7
N.D.
N.D.
N.D.
-0.2
stress
McCas_formate_complete mercury (II) chloride 0.227272727272727 mM
-1.9
N.D.
N.D.
N.D.
+0.0
rich t2
H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
-1.6
N.D.
N.D.
N.D.
-0.3
stress
McCas_H2_complete Benzethonium chloride 0.003125 mM
-2.0
N.D.
N.D.
N.D.
+0.1
nitrogen source
H2 N-free with 10mM Ammonium chloride
-2.0
N.D.
N.D.
N.D.
+0.2
stress
McCas_formate_complete 3-nitroxypropanol 0.000416666666666667 mM
-2.0
N.D.
N.D.
N.D.
+0.2
stress
McCas_H2_complete Nitrofurantoin 0.1 mM
-1.8
N.D.
N.D.
N.D.
+0.4
stress
McCas_H2_complete 5-Fluorouracil 0.00833333333333333 mM
+0.5
N.D.
N.D.
N.D.
-1.9
stress
H2 McCas with 0.1 mM sulfite
-1.7
N.D.
N.D.
N.D.
+0.5
nitrogen source
H2 N-free with 10mM L-Arginine
-1.5
N.D.
N.D.
N.D.
+0.5
stress
McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM
-1.7
N.D.
N.D.
N.D.
+0.9
stress
H2 McCas with 10 mM thiosulfate
-1.2
N.D.
N.D.
N.D.
+0.8
nutrient t2
H2 deepwell acetate FREE
-1.1
N.D.
N.D.
N.D.
+1.0
stress
McCas_formate_complete nitrate 35.7142857142857 mM
+1.2
N.D.
N.D.
N.D.
+0.7
stress
McCas_H2_complete Hydroxylamine 1.04166666666667 mM
+0.1
N.D.
N.D.
N.D.
+1.9
no stress control
McCas_H2_complete no stress control
+1.1
N.D.
N.D.
N.D.
+1.0
stress
McCas_H2_complete 5-Fluorouracil 0.00833333333333333 mM
+1.9
N.D.
N.D.
N.D.
+0.3
stress
McCas_H2_complete Cadmium chloride hemipentahydrate 0.555555555555556 mM
+3.4
N.D.
N.D.
N.D.
+2.6
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