Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS08890 and MMJJ_RS08895 are separated by 129 nucleotidesMMJJ_RS08895 and MMJJ_RS08900 are separated by 21 nucleotidesMMJJ_RS08900 and MMJJ_RS08905 are separated by 59 nucleotidesMMJJ_RS08905 and MMJJ_RS08910 are separated by 22 nucleotides MMJJ_RS08890: MMJJ_RS08890 - NAD(P)/FAD-dependent oxidoreductase, at 1,640,185 to 1,641,636 _RS08890 MMJJ_RS08895: MMJJ_RS08895 - tRNA-Ala, at 1,641,766 to 1,641,839 _RS08895 MMJJ_RS08900: MMJJ_RS08900 - DUF523 and DUF1722 domain-containing protein, at 1,641,861 to 1,642,829 _RS08900 MMJJ_RS08905: MMJJ_RS08905 - YcaO-related McrA-glycine thioamidation protein, at 1,642,889 to 1,644,091 _RS08905 MMJJ_RS08910: MMJJ_RS08910 - DUF167 domain-containing protein, at 1,644,114 to 1,644,419 _RS08910
Group Condition MMJJ_RS08890 MMJJ_RS08895 MMJJ_RS08900 MMJJ_RS08905 MMJJ_RS08910
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.1 N.D. +0.1 -3.1 -0.4
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM +0.2 N.D. +0.1 -3.1 -0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.0 N.D. +0.0 -2.7 -0.3
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM -0.0 N.D. +0.2 -3.0 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.0 N.D. -0.0 -2.4 -0.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.0 N.D. +0.0 -2.6 -0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.0 N.D. -0.0 -2.1 -0.5
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.0 N.D. +0.0 -2.6 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.0 N.D. +0.1 -2.4 -0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.0 N.D. -0.1 -2.1 -0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 N.D. -0.1 -1.6 -0.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 N.D. +0.0 -2.0 -0.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.1 N.D. +0.1 -2.4 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.0 N.D. +0.0 -2.0 -0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 N.D. +0.0 -1.8 -0.5
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.0 N.D. -0.0 -1.6 -0.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.0 N.D. -0.1 -1.8 -0.1
nutrient t1 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -0.1 N.D. +0.0 -1.7 -0.2
hydrogen no stress control H2 no stress control +0.0 N.D. +0.2 -1.4 -0.8
hydrogen no stress control H2 no stress control +0.0 N.D. +0.1 -1.5 -0.6
iron Iron excess (2.5x) , H2 media +0.1 N.D. +0.3 -2.3 +0.1
iron Iron excess (2.5x) , H2 media +0.0 N.D. +0.1 -1.9 +0.1
planktonic H2 pooled triplicate planktonic cells +0.2 N.D. +0.2 -2.1 -0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.1 N.D. +0.1 -1.5 -0.4
nitrogen source H2 N-free with 10mM D-glucosamine +0.0 N.D. +0.2 -1.9 -0.1
nitrogen source H2 N-free with 10mM Ammonium chloride -0.1 N.D. +0.1 -1.8 +0.1
nitrogen source H2 N-free with 10mM Ammonium chloride +0.0 N.D. +0.1 -1.8 +0.1
iron Iron excess (2.5x) , H2 media +0.1 N.D. +0.2 -2.0 +0.1
nitrogen source N2 fixation, H2 media -0.1 N.D. +0.5 -0.8 -0.8
stress McCas_formate_complete Chlorpromazine 0.0166666666666667 mM +0.1 N.D. +0.4 +0.9 +0.5
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