Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS08855 and MMJJ_RS08860 are separated by 109 nucleotidesMMJJ_RS08860 and MMJJ_RS08865 overlap by 4 nucleotidesMMJJ_RS08865 and MMJJ_RS08870 are separated by 131 nucleotidesMMJJ_RS08870 and MMJJ_RS08875 are separated by 83 nucleotides MMJJ_RS08855: MMJJ_RS08855 - cyclic pyranopterin monophosphate synthase MoaC, at 1,632,920 to 1,633,381 _RS08855 MMJJ_RS08860: MMJJ_RS08860 - Rrf2 family transcriptional regulator, at 1,633,491 to 1,633,925 _RS08860 MMJJ_RS08865: MMJJ_RS08865 - class IV adenylate cyclase, at 1,633,922 to 1,634,485 _RS08865 MMJJ_RS08870: MMJJ_RS08870 - 2-isopropylmalate synthase, at 1,634,617 to 1,636,161 _RS08870 MMJJ_RS08875: MMJJ_RS08875 - hypothetical protein, at 1,636,245 to 1,638,089 _RS08875
Group Condition MMJJ_RS08855 MMJJ_RS08860 MMJJ_RS08865 MMJJ_RS08870 MMJJ_RS08875
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -3.1 -0.3 -3.4 -5.3 -0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -3.8 -0.2 -1.1 -5.5 -0.1
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -5.9 -0.4 -0.9 -3.5 +0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -6.3 -0.3 -0.5 -3.5 -0.0
nitrogen source Formate N-free with 2mM Adenosine -4.2 +0.2 -1.5 -4.8 +0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -3.2 -0.2 -3.0 -3.3 -0.2
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -6.3 -0.4 -0.7 -2.4 +0.0
nitrogen source Formate N-free with 2mM Adenosine -4.5 -0.3 -1.9 -2.6 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -5.6 -0.4 -0.5 -2.9 +0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -3.5 -0.2 -0.7 -4.5 +0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -5.4 -0.4 -0.3 -2.8 +0.0
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -6.5 -0.4 -0.7 -1.1 -0.0
iron Iron excess (2.5x) , H2 media -3.6 +0.0 -0.8 -3.9 -0.2
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -4.7 -0.1 -0.5 -2.9 -0.1
nitrogen source formate N-free with 10mM Ammonium chloride -2.2 -0.1 -0.3 -5.4 -0.1
nitrogen source H2 N-free with 2mM Adenosine -2.4 -0.6 -0.5 -4.1 -0.5
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -2.6 -0.5 -0.5 -4.4 +0.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -4.7 +0.0 -0.3 -2.8 -0.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -2.3 -0.2 -0.8 -4.1 -0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -2.3 -0.4 -2.9 -1.9 -0.2
iron control, 1x iron with casaminos and vitamins, H2 media -3.7 +0.1 -0.5 -3.3 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -2.5 -0.1 -1.3 -3.5 -0.1
nitrogen source Formate N-free with 2mM Adenosine -3.8 +0.3 -1.6 -2.1 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -2.7 -0.4 -0.5 -3.5 +0.0
nitrogen source H2 N-free with 2mM Adenosine -2.4 -0.2 -1.0 -3.0 -0.3
nitrogen source H2 N-free with 10mM Ammonium chloride -2.4 +0.1 -0.3 -4.0 -0.1
nitrogen source H2 N-free with 10mM Ammonium chloride -2.3 +0.2 -0.2 -4.0 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -4.7 -0.4 -1.8 +0.5 +0.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -4.3 -0.3 -1.6 +0.5 +0.2
stress McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM -0.8 -0.8 +0.7 -4.6 +0.2
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