Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS08735 and MMJJ_RS08740 are separated by 47 nucleotidesMMJJ_RS08740 and MMJJ_RS08745 are separated by 17 nucleotidesMMJJ_RS08745 and MMJJ_RS08750 overlap by 12 nucleotidesMMJJ_RS08750 and MMJJ_RS08755 are separated by 8 nucleotides MMJJ_RS08735: MMJJ_RS08735 - hypothetical protein, at 1,609,374 to 1,610,123 _RS08735 MMJJ_RS08740: MMJJ_RS08740 - class I SAM-dependent methyltransferase, at 1,610,171 to 1,610,845 _RS08740 MMJJ_RS08745: MMJJ_RS08745 - glycosyltransferase family 9 protein, at 1,610,863 to 1,611,921 _RS08745 MMJJ_RS08750: MMJJ_RS08750 - glycosyltransferase, at 1,611,910 to 1,613,139 _RS08750 MMJJ_RS08755: MMJJ_RS08755 - ADP-glyceromanno-heptose 6-epimerase, at 1,613,148 to 1,614,068 _RS08755
Group Condition MMJJ_RS08735 MMJJ_RS08740 MMJJ_RS08745 MMJJ_RS08750 MMJJ_RS08755
attachment assay; attached sample McCas-formate glass attached -0.2 -0.1 +0.0 -1.2 -0.6
nitrogen source H2 N-free with 2mM Adenosine +0.2 -0.2 +0.2 -1.7 -0.3
nitrogen source Formate N-free with 2mM Adenosine +0.1 +0.3 +0.2 -1.7 -0.8
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.2 +0.1 +0.1 +0.8 -2.7
nitrogen source Formate N-free with 2mM Adenosine +0.2 +0.1 +0.7 -1.8 -0.8
nitrogen source Formate N-free with 2mM Adenosine +0.3 +0.2 +0.3 -2.1 -0.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 +0.2 -0.0 +0.9 -2.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 +0.3 +0.0 +0.5 -1.9
iron Iron excess (2.5x) , H2 media +0.1 +0.1 +0.2 -1.3 -0.4
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.2 -0.2 +0.1 +0.7 -1.1
stress McCas_formate_complete sodium nitrite 0.125 mM +0.0 -0.2 -0.0 +1.2 -0.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.1 -0.1 -0.0 +1.5 -0.9
stress McCas_formate_complete sodium nitrite 0.125 mM +0.0 -0.0 +0.1 +1.3 -0.7
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.0 -0.1 +0.0 +1.8 -0.9
stress McCas_formate_complete Spiramycin 0.1 mM +0.2 -0.1 +0.2 +1.1 -0.6
no stress control McCas_formate_complete no stress control -0.1 -0.1 -0.0 +1.7 -0.2
no stress control McCas_formate_complete no stress control -0.2 -0.1 -0.0 +1.7 -0.2
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM +0.2 -0.6 -0.1 +2.0 -0.2
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM -0.1 -0.2 +0.2 +1.9 -0.3
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.0 -0.2 -0.1 +1.2 +0.6
no stress control McCas_formate_complete no stress control +0.0 -0.2 -0.0 +2.0 -0.2
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.0 -0.1 -0.1 +1.2 +0.6
stress McCas_formate_complete phosphite 25 mM +0.1 -0.2 -0.1 +1.9 -0.1
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.0 -0.1 -0.1 +1.3 +0.7
no stress control McCas_formate_complete no stress control -0.0 -0.2 +0.1 +1.6 +0.3
no stress control McCas_formate_complete no stress control +0.1 -0.2 +0.2 +1.9 +0.1
stress McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM +0.2 +0.1 +0.1 +1.6 +0.2
stress McCas_formate_complete phosphite 25 mM -0.1 -0.1 +0.1 +2.0 +0.2
stress McCas_formate_complete Antimycin A 0.01 mM +0.4 -0.1 +0.1 +1.8 -0.0
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM +0.1 +0.2 +0.5 +1.5 +0.1
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