Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS08710 and MMJJ_RS08715 are separated by 33 nucleotidesMMJJ_RS08715 and MMJJ_RS08720 are separated by 15 nucleotidesMMJJ_RS08720 and MMJJ_RS08725 are separated by 171 nucleotidesMMJJ_RS08725 and MMJJ_RS08730 are separated by 142 nucleotides MMJJ_RS08710: MMJJ_RS08710 - UTP--glucose-1-phosphate uridylyltransferase GalU, at 1,603,393 to 1,604,241 _RS08710 MMJJ_RS08715: MMJJ_RS08715 - NAD-dependent epimerase/dehydratase family protein, at 1,604,275 to 1,605,150 _RS08715 MMJJ_RS08720: MMJJ_RS08720 - UDP-glucose/GDP-mannose dehydrogenase family protein, at 1,605,166 to 1,606,488 _RS08720 MMJJ_RS08725: MMJJ_RS08725 - flippase, at 1,606,660 to 1,608,174 _RS08725 MMJJ_RS08730: MMJJ_RS08730 - glycosyltransferase family 4 protein, at 1,608,317 to 1,609,381 _RS08730
Group Condition MMJJ_RS08710 MMJJ_RS08715 MMJJ_RS08720 MMJJ_RS08725 MMJJ_RS08730
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM -3.3 -2.3 -1.1 -1.9 +0.1
attachment assay; attached sample McCas-formate glass attached -1.6 -1.4 -1.6 -1.8 -1.1
nitrogen source Formate N-free with 2mM Adenosine -2.4 -1.1 -1.0 -0.9 -1.3
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM -1.9 -1.8 -0.8 -1.9 +0.2
attachment assay; attached sample McCas-formate glass attached -1.2 -1.6 -1.1 -0.9 -1.2
stress Formate 100 mM hypophosphite -1.2 -1.5 -1.4 -1.7 -0.2
nitrogen source H2 N-free with 10mM D-glucosamine -2.3 -1.6 -1.4 -0.8 +0.2
biofilm H2 pooled triplicate biofilm cells -1.4 -0.9 -1.3 -2.0 -0.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE -1.6 -2.4 -0.7 -1.6 +0.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE -1.4 -2.1 -0.9 -1.8 +0.9
stress McCas_formate_complete Rifampicin 0.25 mM -0.8 -2.0 -1.3 -1.4 +0.5
stress McCas_formate_complete Chlorpromazine 0.0166666666666667 mM -1.1 -3.0 +0.3 +3.9 +0.1
stress McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM +2.0 +0.2 +1.0 +1.6 +0.9
stress McCas_formate_complete phosphite 25 mM +1.3 +1.1 +1.5 +0.1 +1.8
stress McCas_formate_complete Fluorophosphate 31.25 mM +2.7 -0.0 +1.0 +0.8 +1.4
no stress control McCas_formate_complete no stress control +1.0 +0.9 +1.5 +1.6 +1.3
no stress control McCas_formate_complete no stress control +1.3 +0.5 +1.5 +1.9 +1.1
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM +1.6 +1.1 +1.4 +2.0 +0.3
no stress control McCas_formate_complete no stress control +1.2 +0.8 +1.3 +2.5 +0.6
stress McCas_formate_complete Irgasan 0.0125 mM +2.5 +0.1 +0.8 +1.7 +1.3
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM +2.5 +0.4 +0.8 +1.5 +1.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.6 +1.1 +1.9 +1.6 +1.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.7 +0.9 +1.7 +2.0 +1.4
no stress control McCas_formate_complete no stress control +1.9 +1.2 +1.5 +1.4 +1.1
no stress control McCas_formate_complete no stress control +1.1 +0.6 +1.9 +2.2 +1.2
stress McCas_formate_complete 3-nitroxypropanol 0.00078125 mM +1.7 +0.2 +1.7 +2.3 +1.2
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM +2.4 +0.4 +0.8 +2.6 +1.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.7 +1.2 +1.9 +2.3 +1.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +1.3 +1.6 +2.2 +2.0 +1.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +1.8 +2.0 +3.6 +3.9 +1.7
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