Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS08530 and MMJJ_RS08535 are separated by 50 nucleotidesMMJJ_RS08535 and MMJJ_RS08540 are separated by 52 nucleotidesMMJJ_RS08540 and MMJJ_RS08545 are separated by 42 nucleotidesMMJJ_RS08545 and MMJJ_RS08550 are separated by 342 nucleotides MMJJ_RS08530: MMJJ_RS08530 - RNA-binding transcriptional accessory protein, at 1,568,708 to 1,570,849 _RS08530 MMJJ_RS08535: MMJJ_RS08535 - NOL1/NOP2/sun family putative RNA methylase, at 1,570,900 to 1,571,697 _RS08535 MMJJ_RS08540: MMJJ_RS08540 - archaeosortase family protein ArtE, at 1,571,750 to 1,572,223 _RS08540 MMJJ_RS08545: MMJJ_RS08545 - thiamine-phosphate kinase, at 1,572,266 to 1,573,213 _RS08545 MMJJ_RS08550: MMJJ_RS08550 - B12-binding domain-containing radical SAM protein, at 1,573,556 to 1,574,872 _RS08550
Group Condition MMJJ_RS08530 MMJJ_RS08535 MMJJ_RS08540 MMJJ_RS08545 MMJJ_RS08550
stress McCas_formate_complete Ticarcillin 12.5 mM -0.2 -3.9 -0.6 N.D. -0.3
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM -0.2 -4.7 -0.0 N.D. -0.0
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.0 -4.4 -0.1 N.D. -0.0
attachment assay; attached sample McCas-formate glass attached +0.3 -5.1 +0.0 N.D. +0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.0 -4.5 -0.1 N.D. +0.0
stress McCas_formate_complete Fluorophosphate 31.25 mM -0.6 -3.8 +0.1 N.D. -0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.1 -4.3 -0.0 N.D. +0.0
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM -0.4 -4.1 +0.1 N.D. -0.0
iron Iron excess (2.5x) , H2 media +0.1 -4.2 +0.1 N.D. -0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -4.1 -0.0 N.D. -0.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.0 -4.3 -0.0 N.D. +0.1
stress McCas_formate_complete sulfate 125 mM -0.5 -3.2 -0.1 N.D. -0.3
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.1 -4.3 +0.1 N.D. -0.1
stress McCas_formate_complete Nitrofurantoin 0.025 mM -0.3 -4.0 +0.3 N.D. -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.2 -3.6 -0.1 N.D. -0.1
stress McCas_formate_complete Spiramycin 0.1 mM -0.1 -3.8 +0.2 N.D. -0.3
iron control, 1x iron with casaminos and vitamins, H2 media +0.1 -4.2 +0.2 N.D. -0.0
stress McCas_formate_complete hypophosphite 62.5 mM -0.2 -3.9 +0.1 N.D. +0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.0 -4.0 +0.0 N.D. +0.1
iron Iron excess (2.5x) , H2 media +0.2 -4.1 -0.0 N.D. +0.0
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.0 -4.0 +0.0 N.D. +0.1
iron Iron limitation (0.2x), H2 media +0.2 -4.1 +0.0 N.D. -0.0
nitrogen source H2 N-free with 10mM Ammonium chloride +0.2 -3.8 +0.2 N.D. +0.0
planktonic H2 pooled triplicate planktonic cells +0.2 -3.8 +0.2 N.D. +0.1
stress McCas_formate_complete selenate 31.25 mM -0.3 -3.3 +0.2 N.D. +0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.1 -3.7 +0.2 N.D. +0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.2 -3.8 +0.3 N.D. +0.1
nitrogen source H2 N-free with 10mM D-glucosamine +0.2 -3.7 +0.2 N.D. +0.2
nitrogen source H2 N-free with 10mM D-glucosamine +0.1 -3.6 +0.1 N.D. +0.4
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.3 -3.5 -0.1 N.D. +0.9
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