Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS08505 and MMJJ_RS08510 are separated by 186 nucleotidesMMJJ_RS08510 and MMJJ_RS08515 are separated by 73 nucleotidesMMJJ_RS08515 and MMJJ_RS08520 are separated by 114 nucleotidesMMJJ_RS08520 and MMJJ_RS08525 are separated by 41 nucleotides MMJJ_RS08505: MMJJ_RS08505 - tRNA-intron lyase, at 1,564,967 to 1,565,500 _RS08505 MMJJ_RS08510: MMJJ_RS08510 - peptide chain release factor aRF-1, at 1,565,687 to 1,566,946 _RS08510 MMJJ_RS08515: MMJJ_RS08515 - secondary thiamine-phosphate synthase enzyme YjbQ, at 1,567,020 to 1,567,418 _RS08515 MMJJ_RS08520: MMJJ_RS08520 - peptidylprolyl isomerase, at 1,567,533 to 1,568,021 _RS08520 MMJJ_RS08525: MMJJ_RS08525 - peptidylprolyl isomerase, at 1,568,063 to 1,568,665 _RS08525
Group Condition MMJJ_RS08505 MMJJ_RS08510 MMJJ_RS08515 MMJJ_RS08520 MMJJ_RS08525
nitrogen source H2 N-free with 2mM Adenosine N.D. N.D. -0.1 -2.6 -0.2
stress McCas_formate_complete Irgasan 0.0125 mM N.D. N.D. -1.1 -0.8 -0.5
stress McCas_formate_complete hypophosphite 62.5 mM N.D. N.D. -0.5 -0.4 -1.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. N.D. +0.1 -1.3 -0.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. N.D. -0.2 -1.1 -0.6
no stress control McCas_formate_complete no stress control N.D. N.D. -0.7 -0.1 -1.1
stress McCas_formate_complete Nitrofurantoin 0.025 mM N.D. N.D. -0.6 -1.0 -0.1
planktonic H2 pooled triplicate planktonic cells N.D. N.D. -0.2 -0.7 -0.6
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE N.D. N.D. -0.8 -0.1 -0.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. N.D. -0.0 -1.0 -0.5
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. N.D. +0.0 -1.2 -0.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. N.D. +0.1 -1.3 -0.2
stress Formate 100 mM hypophosphite N.D. N.D. +0.2 -1.6 +0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. N.D. +0.2 -0.8 -0.4
nitrogen source Formate N-free with 2mM Adenosine N.D. N.D. +1.2 -2.0 -0.1
nutrient t1 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. N.D. +0.3 -1.0 -0.2
rich t1 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. N.D. +0.3 -0.7 -0.4
stress McCas_formate_complete Irgasan 0.0125 mM N.D. N.D. -0.7 -0.3 +0.4
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 N.D. N.D. +0.3 -1.4 +0.5
stress McCas_formate_complete Novobiocin 0.0125 mM N.D. N.D. -1.0 +1.0 -0.4
nitrogen source N2 fixation, H2 media N.D. N.D. -0.6 +0.8 -0.6
nitrogen source H2 N-free with 2mM Adenosine N.D. N.D. -0.1 -0.9 +0.6
stress McCas_formate_complete Fluorophosphate 31.25 mM N.D. N.D. -0.9 +0.4 +0.2
stress McCas_formate_complete sulfate 125 mM N.D. N.D. -0.4 +1.2 -0.7
stress Formate 100 mM hypophosphite N.D. N.D. +1.6 -0.6 +0.2
stress Formate 100 mM hypophosphite N.D. N.D. +1.6 -0.6 +0.2
nitrogen source H2 N-free with 10mM D-glucosamine N.D. N.D. -0.0 +1.4 -0.1
stress McCas_formate_complete silver chloride 0.125 mM N.D. N.D. -0.3 +1.2 +0.5
stress McCas_formate_complete Spiramycin 0.1 mM N.D. N.D. +0.6 +0.4 +0.5
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM N.D. N.D. +0.5 +0.8 +0.3
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