Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS08395 and MMJJ_RS08400 are separated by 12 nucleotidesMMJJ_RS08400 and MMJJ_RS08405 are separated by 227 nucleotidesMMJJ_RS08405 and MMJJ_RS08410 are separated by 59 nucleotidesMMJJ_RS08410 and MMJJ_RS08415 are separated by 172 nucleotides MMJJ_RS08395: MMJJ_RS08395 - CoB--CoM heterodisulfide reductase subunit B, at 1,544,920 to 1,545,825 _RS08395 MMJJ_RS08400: MMJJ_RS08400 - CoB--CoM heterodisulfide reductase subunit C, at 1,545,838 to 1,546,416 _RS08400 MMJJ_RS08405: MMJJ_RS08405 - nickel-dependent hydrogenase large subunit, at 1,546,644 to 1,547,771 _RS08405 MMJJ_RS08410: MMJJ_RS08410 - CBS domain-containing protein, at 1,547,831 to 1,548,193 _RS08410 MMJJ_RS08415: MMJJ_RS08415 - corrinoid protein, at 1,548,366 to 1,549,012 _RS08415
Group Condition MMJJ_RS08395 MMJJ_RS08400 MMJJ_RS08405 MMJJ_RS08410 MMJJ_RS08415
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -0.0 -0.8 -4.1 +0.2 -0.1
nitrogen source H2 N-free with 2mM Adenosine +0.1 -1.3 -1.2 -2.0 -0.3
nitrogen source Formate N-free with 2mM Adenosine +0.3 -1.1 -3.3 -0.6 +0.4
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM -0.2 -1.1 -0.4 -2.2 +0.1
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.0 -0.5 -2.7 -0.4 +0.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.1 +0.6 -3.9 -0.5 +0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.2 +0.3 -3.7 -0.7 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.1 +0.5 -4.0 -0.2 +0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.0 -0.2 -2.5 -0.7 -0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.1 -0.6 -2.8 -0.2 -0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +0.0 -0.7 -2.3 -0.5 -0.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.0 +0.3 -3.3 -0.3 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.0 -0.1 -3.1 -0.3 +0.1
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.1 -0.4 -2.5 -0.4 +0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.1 -0.3 -2.6 -0.3 -0.1
iron control, 1x iron with casaminos and vitamins, H2 media +0.0 -0.7 -2.4 -0.2 +0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.1 -0.4 -2.5 -0.2 -0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.1 -0.4 -2.7 -0.1 +0.0
iron control, 1x iron with casaminos and vitamins, H2 media +0.1 -0.3 -2.2 -0.5 -0.1
stress McCas_formate_complete Nitrofurantoin 0.025 mM -0.3 -1.7 -1.0 +0.1 -0.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.1 -0.1 -2.6 -0.2 +0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 +0.1 -3.1 +0.2 +0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 +0.3 -2.1 -0.6 -0.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE -0.0 +0.4 -3.0 -0.2 +0.2
nitrogen source Formate N-free with 2mM Adenosine -0.1 -0.5 -2.6 +0.8 +0.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.0 +0.6 -3.1 -0.2 +0.3
iron Iron excess (2.5x) , H2 media -0.0 +0.2 -2.3 +0.6 +0.2
nitrogen source Formate N-free with 2mM Adenosine +0.2 +0.9 -2.8 +0.5 +0.4
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM +0.5 +1.6 -1.4 +0.0 +0.7
nitrogen source N2 fixation, H2 media -0.1 +0.3 +0.6 +2.2 +1.0
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