Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS08050 and MMJJ_RS08055 are separated by 289 nucleotidesMMJJ_RS08055 and MMJJ_RS08060 are separated by 91 nucleotidesMMJJ_RS08060 and MMJJ_RS08065 are separated by 148 nucleotidesMMJJ_RS08065 and MMJJ_RS08070 are separated by 97 nucleotides MMJJ_RS08050: MMJJ_RS08050 - ABC transporter substrate-binding protein, at 1,473,578 to 1,474,342 _RS08050 MMJJ_RS08055: MMJJ_RS08055 - methanogenesis marker 14 protein, at 1,474,632 to 1,476,110 _RS08055 MMJJ_RS08060: MMJJ_RS08060 - DNA repair and recombination protein RadA, at 1,476,202 to 1,477,170 _RS08060 MMJJ_RS08065: MMJJ_RS08065 - precorrin-6Y C5,15-methyltransferase (decarboxylating) subunit CbiT, at 1,477,319 to 1,477,864 _RS08065 MMJJ_RS08070: MMJJ_RS08070 - alpha-glucan family phosphorylase, at 1,477,962 to 1,479,524 _RS08070
Group Condition MMJJ_RS08050 MMJJ_RS08055 MMJJ_RS08060 MMJJ_RS08065 MMJJ_RS08070
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.0 -2.8 -1.2 -4.6 +0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +0.0 -2.7 -0.9 -4.8 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.1 -2.7 -1.0 -4.7 +0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.0 -3.1 -0.9 -4.3 -0.1
iron Iron limitation (0.2x), formate media -0.1 -3.0 -0.0 -5.1 -0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.0 -2.7 -0.8 -4.3 +0.0
iron Iron limitation (0.2x), formate media -0.1 -3.5 -0.0 -4.3 +0.2
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.0 -2.6 -0.7 -4.1 -0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.1 -2.4 -0.6 -4.2 -0.1
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.0 -2.7 -0.6 -3.8 +0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.1 -2.4 -0.6 -4.0 -0.1
nutrient t1 formate McAA pyroxidine HCl dropout +0.0 -3.6 -0.1 -3.3 +0.1
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.0 -2.3 -0.6 -3.9 -0.0
iron Iron limitation (0.2x), formate media -0.0 -3.2 -0.1 -3.5 +0.2
nutrient t1 formate McAA lipolic dropout +0.1 -3.4 -0.2 -2.8 -0.0
nutrient t1 formate McAA B12 dropout -0.0 -2.8 -0.2 -3.0 -0.1
nutrient t1 formate McAA p-aminobenzoic dropout -0.1 -3.0 -0.2 -2.7 -0.1
stress Formate 50 mM hypophosphite +0.1 -3.3 -0.9 -2.0 +0.0
hydrogen no stress control H2 no stress control +0.0 -4.1 -1.6 -0.2 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.2 -2.4 -0.1 -3.3 -0.2
nitrogen source Formate N-free with 2mM Adenosine +0.2 -2.0 +0.1 -4.1 +0.5
nitrogen source Formate N-free with 2mM Adenosine +0.1 -1.9 +0.3 -3.2 +0.9
planktonic H2 pooled triplicate planktonic cells +0.4 -2.7 -2.1 +2.4 +0.0
biofilm H2 pooled triplicate biofilm cells +0.2 -2.7 -1.1 +1.8 +0.2
stress McCas_formate_complete Ticarcillin 12.5 mM -0.1 -1.2 -2.1 +3.0 -0.0
stress McCas_formate_complete Irgasan 0.0125 mM -0.1 -0.9 -1.6 +3.1 +0.4
stress McCas_formate_complete Nitrofurantoin 0.025 mM -0.2 -1.2 -1.5 +3.1 +0.8
stress McCas_formate_complete Nitrofurantoin 0.025 mM +0.2 -1.2 -1.7 +3.4 +0.3
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM +0.0 -1.4 -0.9 +4.6 +0.0
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM -0.0 -0.9 -1.1 +5.4 +0.2
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