Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS08015 and MMJJ_RS08020 are separated by 28 nucleotidesMMJJ_RS08020 and MMJJ_RS08025 are separated by 72 nucleotidesMMJJ_RS08025 and MMJJ_RS08030 are separated by 94 nucleotidesMMJJ_RS08030 and MMJJ_RS08035 are separated by 13 nucleotides MMJJ_RS08015: MMJJ_RS08015 - HAD family hydrolase, at 1,468,117 to 1,468,566 _RS08015 MMJJ_RS08020: MMJJ_RS08020 - nucleotidyltransferase domain-containing protein, at 1,468,595 to 1,469,653 _RS08020 MMJJ_RS08025: MMJJ_RS08025 - hypothetical protein, at 1,469,726 to 1,470,028 _RS08025 MMJJ_RS08030: MMJJ_RS08030 - hypothetical protein, at 1,470,123 to 1,470,770 _RS08030 MMJJ_RS08035: MMJJ_RS08035 - cobalt-precorrin-7 (C(5))-methyltransferase, at 1,470,784 to 1,471,443 _RS08035
Group Condition MMJJ_RS08015 MMJJ_RS08020 MMJJ_RS08025 MMJJ_RS08030 MMJJ_RS08035
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.2 -4.0 -3.7 -0.4 -4.4
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.2 -4.2 -1.6 -0.2 -4.4
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.2 -4.1 -1.3 -0.3 -4.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.2 -4.3 -1.1 -0.2 -4.5
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.2 -4.0 +0.6 -0.0 -5.4
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.0 -4.1 -0.5 -0.2 -3.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.1 -4.2 -0.3 -0.0 -3.7
iron Iron excess (2.5x) , H2 media -0.5 -3.5 -0.1 +0.4 -4.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.1 -3.8 -0.2 -0.1 -4.1
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.0 -4.2 +0.2 +0.0 -4.0
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.0 -3.7 +1.2 -0.0 -5.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -3.8 +0.7 -0.1 -4.8
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.0 -4.0 +1.0 -0.1 -4.8
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.1 -3.7 +0.1 +0.0 -4.2
iron control, 1x iron with casaminos and vitamins, H2 media -0.4 -3.4 +0.1 +0.2 -4.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.0 -3.7 +0.5 -0.1 -4.5
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.2 -4.1 +0.0 -0.1 -3.5
nitrogen source H2 N-free with 2mM Adenosine +0.1 -2.8 -1.1 +0.1 -3.7
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.1 -3.1 -0.0 +0.3 -4.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 -3.2 +0.5 -0.1 -4.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.0 -3.5 +0.3 +0.0 -4.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 -3.0 +0.6 -0.1 -4.6
nitrogen source Formate N-free with 2mM Adenosine -0.1 -3.6 +0.1 +0.5 -3.8
nitrogen source Formate N-free with 2mM Adenosine +0.1 -4.2 -0.3 +0.9 -3.4
nitrogen source H2 N-free with 2g/L casaminos -0.0 -3.0 +0.4 +0.2 -4.5
nitrogen source H2 N-free with 10mM Ammonium chloride -0.3 -2.9 +0.6 +0.1 -3.9
iron Iron excess (2.5x) , H2 media +0.2 -3.1 +0.7 +0.2 -4.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -2.1 +0.8 -0.2 -4.6
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE -0.1 -3.3 +1.2 -0.3 -3.6
nitrogen source H2 N-free with 10mM D-glucosamine +0.0 -3.0 +0.8 +0.2 -3.8
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