Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS07695 and MMJJ_RS07700 are separated by 67 nucleotidesMMJJ_RS07700 and MMJJ_RS07705 are separated by 16 nucleotidesMMJJ_RS07705 and MMJJ_RS07710 are separated by 125 nucleotidesMMJJ_RS07710 and MMJJ_RS07715 are separated by 62 nucleotides MMJJ_RS07695: MMJJ_RS07695 - glycoside hydrolase family 15 protein, at 1,411,146 to 1,413,011 _RS07695 MMJJ_RS07700: MMJJ_RS07700 - glycoside hydrolase family 57 protein, at 1,413,079 to 1,414,302 _RS07700 MMJJ_RS07705: MMJJ_RS07705 - GTP-binding protein EngB, at 1,414,319 to 1,415,011 _RS07705 MMJJ_RS07710: MMJJ_RS07710 - 50S ribosomal protein L16, at 1,415,137 to 1,415,658 _RS07710 MMJJ_RS07715: MMJJ_RS07715 - UbiD family decarboxylase, at 1,415,721 to 1,416,998 _RS07715
Group Condition MMJJ_RS07695 MMJJ_RS07700 MMJJ_RS07705 MMJJ_RS07710 MMJJ_RS07715
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM +0.1 -0.1 -2.6 N.D. -4.7
stress McCas_formate_complete Rifampicin 0.25 mM -0.0 +0.1 -2.5 N.D. -4.6
stress McCas_formate_complete silver chloride 0.125 mM +0.1 +0.1 -2.5 N.D. -4.6
nitrogen source H2 N-free with 10mM Ammonium chloride +0.2 +0.0 -3.7 N.D. -3.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.0 -0.4 -3.3 N.D. -2.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -0.5 -3.1 N.D. -2.4
nitrogen source H2 N-free with 10mM D-glucosamine +0.4 -0.2 -3.2 N.D. -3.0
planktonic H2 pooled triplicate planktonic cells +0.4 +0.5 -3.5 N.D. -3.3
iron control, 1x iron with casaminos and vitamins, H2 media +0.2 +0.0 -4.4 N.D. -1.8
nitrogen source H2 N-free with 2g/L casaminos +0.3 -0.1 -4.6 N.D. -1.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.1 -0.2 -3.3 N.D. -2.4
nutrient t1 formate McAA pyroxidine HCl dropout +0.2 -0.1 -4.7 N.D. -1.0
stress McCas_formate_complete Spiramycin 0.1 mM +0.2 +0.1 -2.2 N.D. -3.7
stress McCas_formate_complete Ticarcillin 12.5 mM -0.1 -0.4 -1.3 N.D. -3.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.1 -0.1 -2.8 N.D. -2.7
iron Iron limitation (0.2x), H2 media +0.3 -0.1 -3.5 N.D. -2.1
nutrient t1 formate McAA pantothenate dropout +0.2 -0.0 -4.2 N.D. -1.4
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM +0.0 +0.1 -2.3 N.D. -3.1
nitrogen source H2 N-free with 2g/L casaminos +0.3 -0.0 -3.8 N.D. -1.9
iron control, 1x iron with casaminos and vitamins, H2 media +0.4 -0.0 -3.3 N.D. -2.3
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.4 +0.1 -3.8 N.D. -1.8
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.2 -0.4 -3.1 N.D. -1.8
nutrient t1 formate McAA biotin dropout +0.2 -0.1 -3.5 N.D. -1.7
iron Iron excess (2.5x) , H2 media +0.4 +0.1 -4.3 N.D. -1.3
nutrient t1 formate vitamin complete +0.2 -0.1 -3.8 N.D. -1.3
nutrient t1 formate McAA pyroxidine HCl dropout +0.2 +0.1 -3.4 N.D. -1.8
nitrogen source H2 N-free with 2g/L casaminos +0.2 -0.2 -3.3 N.D. -1.7
biofilm H2 pooled triplicate biofilm cells +0.3 +0.3 -3.2 N.D. -2.4
iron Iron excess (2.5x) , H2 media +0.5 +0.1 -3.6 N.D. -1.8
nitrogen source H2 N-free with 2mM Adenosine +0.3 -0.2 +3.5 N.D. -2.2
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