Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS07365 and MMJJ_RS07370 are separated by 15 nucleotidesMMJJ_RS07370 and MMJJ_RS07375 are separated by 138 nucleotidesMMJJ_RS07375 and MMJJ_RS07380 are separated by 90 nucleotidesMMJJ_RS07380 and MMJJ_RS07385 are separated by 163 nucleotides MMJJ_RS07365: MMJJ_RS07365 - CatB-related O-acetyltransferase, at 1,352,505 to 1,353,185 _RS07365 MMJJ_RS07370: MMJJ_RS07370 - tRNA 4-thiouridine(8) synthase ThiI, at 1,353,201 to 1,354,352 _RS07370 MMJJ_RS07375: MMJJ_RS07375 - tRNA 2-thiocytidine biosynthesis TtcA family protein, at 1,354,491 to 1,355,465 _RS07375 MMJJ_RS07380: MMJJ_RS07380 - sulfide-dependent adenosine diphosphate thiazole synthase, at 1,355,556 to 1,356,344 _RS07380 MMJJ_RS07385: MMJJ_RS07385 - ribosomal protein S18-alanine N-acetyltransferase, at 1,356,508 to 1,356,993 _RS07385
Group Condition MMJJ_RS07365 MMJJ_RS07370 MMJJ_RS07375 MMJJ_RS07380 MMJJ_RS07385
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.2 -2.2 -4.1 -5.5 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.2 -1.7 -4.2 -5.1 +0.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.1 -1.8 -3.4 -4.7 +0.3
iron Iron excess (2.5x) , H2 media +0.2 -3.6 -2.6 -3.3 +0.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE -0.0 -2.4 -2.1 -4.5 +0.5
nitrogen source H2 N-free with 2g/L casaminos +0.2 -2.7 -2.4 -4.0 +0.3
nitrogen source H2 N-free with 10mM Ammonium chloride +0.2 -2.6 -2.0 -4.2 +0.2
iron Iron excess (2.5x) , H2 media +0.3 -1.7 -3.2 -3.9 +0.2
iron control, 1x iron with casaminos and vitamins, H2 media +0.4 -2.4 -2.8 -3.6 +0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.0 -1.9 -2.2 -4.5 +0.5
nutrient t1 formate McAA thiamine dropout +0.2 -2.2 -1.4 -4.7 +0.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.4 -2.7 -2.6 -3.5 +0.4
iron control, 1x iron with casaminos and vitamins, H2 media +0.3 -1.6 -2.7 -4.3 +0.4
iron Iron limitation (0.2x), H2 media +0.3 -2.4 -2.0 -4.0 +0.2
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.2 -1.9 -2.6 -3.9 +0.3
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.0 -1.6 -1.3 -5.0 +0.2
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.4 -1.9 -2.6 -3.6 +0.3
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.2 -1.2 -2.2 -4.7 +0.2
iron Iron excess (2.5x) , H2 media +0.4 -2.1 -2.6 -3.5 +0.2
nutrient t1 formate McAA thiamine dropout +0.2 -1.5 -1.7 -4.6 +0.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.2 -1.0 -2.3 -4.6 +0.2
nitrogen source H2 N-free with 10mM Ammonium chloride +0.2 -2.0 -1.9 -4.0 +0.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.3 -1.0 -2.4 -4.3 +0.2
nutrient t1 formate McAA folic dropout +0.2 -2.4 -1.2 -3.8 +0.2
nitrogen source H2 N-free with 2g/L casaminos +0.6 -2.5 -2.3 -2.8 +0.3
nutrient t1 formate vitamin complete +0.2 -2.5 -1.3 -3.4 +0.2
nitrogen source H2 N-free with 10mM Ammonium chloride +0.3 -1.8 -1.8 -3.6 +0.2
nitrogen source H2 N-free with 2g/L casaminos +0.5 -1.9 -1.6 -3.7 +0.2
iron control, 1x iron with casaminos and vitamins, H2 media +0.3 -1.4 -1.9 -3.7 +0.3
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.6 -2.3 -3.9 +1.0 +0.5
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