Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS06615 and MMJJ_RS06620 are separated by 20 nucleotidesMMJJ_RS06620 and MMJJ_RS06625 are separated by 16 nucleotidesMMJJ_RS06625 and MMJJ_RS06630 are separated by 23 nucleotidesMMJJ_RS06630 and MMJJ_RS06635 are separated by 18 nucleotides MMJJ_RS06615: MMJJ_RS06615 - pyruvate synthase subunit PorD, at 1,221,918 to 1,222,175 _RS06615 MMJJ_RS06620: MMJJ_RS06620 - pyruvate synthase subunit PorA, at 1,222,196 to 1,223,359 _RS06620 MMJJ_RS06625: MMJJ_RS06625 - pyruvate synthase subunit PorB, at 1,223,376 to 1,224,272 _RS06625 MMJJ_RS06630: MMJJ_RS06630 - 4Fe-4S dicluster domain-containing protein, at 1,224,296 to 1,224,799 _RS06630 MMJJ_RS06635: MMJJ_RS06635 - 4Fe-4S binding protein, at 1,224,818 to 1,225,234 _RS06635
Group Condition MMJJ_RS06615 MMJJ_RS06620 MMJJ_RS06625 MMJJ_RS06630 MMJJ_RS06635
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. N.D. -3.7 -3.6 -2.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. N.D. -4.1 -3.1 -2.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. N.D. -3.8 -3.2 -2.3
nitrogen source Formate N-free with 2mM Adenosine N.D. N.D. -2.6 -2.6 -3.3
nitrogen source Formate N-free with 2mM Adenosine N.D. N.D. -3.6 -2.2 -1.9
nitrogen source Formate N-free with 2mM Adenosine N.D. N.D. -3.1 -1.9 -2.5
nutrient t1 formate McAA thiamine dropout N.D. N.D. -5.4 -0.5 -1.4
nutrient t1 formate McAA thiamine dropout N.D. N.D. -5.2 -0.7 -1.3
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 N.D. N.D. -3.5 -0.4 -3.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE N.D. N.D. -2.9 -0.9 -3.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE N.D. N.D. -2.9 -0.8 -3.3
nutrient t1 formate McAA folic dropout N.D. N.D. -5.4 -0.4 -1.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE N.D. N.D. -2.9 -0.8 -3.2
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. N.D. -5.1 -1.0 -0.7
iron Iron limitation (0.2x), formate media N.D. N.D. -5.4 -0.3 -1.1
nutrient t1 formate McAA p-aminobenzoic dropout N.D. N.D. -5.3 -0.3 -1.1
nutrient t1 formate McAA pyroxidine HCl dropout N.D. N.D. -5.3 -0.2 -1.0
nutrient t1 formate vitamin complete N.D. N.D. -5.3 -0.2 -1.0
nutrient t1 formate McAA nicotinic dropout N.D. N.D. -5.4 -0.2 -0.9
nutrient t1 formate McAA biotin dropout N.D. N.D. -5.2 -0.2 -1.1
nutrient t1 formate McAA riboflavin dropout N.D. N.D. -5.3 -0.1 -1.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. N.D. -4.9 -1.0 -0.6
nutrient t1 formate McAA pantothenate dropout N.D. N.D. -5.3 -0.1 -1.0
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. N.D. -4.8 -1.0 -0.5
nutrient t1 formate McAA p-aminobenzoic dropout N.D. N.D. -5.2 -0.2 -1.0
nutrient t1 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE N.D. N.D. -4.5 -0.5 -1.3
nutrient t1 formate vitamin complete N.D. N.D. -5.3 -0.1 -0.9
nutrient t1 formate McAA nicotinic dropout N.D. N.D. -5.3 -0.0 -0.8
nutrient t1 formate McAA riboflavin dropout N.D. N.D. -5.3 -0.1 -0.8
iron Iron limitation (0.2x), H2 media N.D. N.D. -5.1 -0.6 -0.4
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