Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS06575 and MMJJ_RS06580 are separated by 295 nucleotidesMMJJ_RS06580 and MMJJ_RS06585 are separated by 55 nucleotidesMMJJ_RS06585 and MMJJ_RS06590 are separated by 223 nucleotidesMMJJ_RS06590 and MMJJ_RS06595 are separated by 179 nucleotides MMJJ_RS06575: MMJJ_RS06575 - prephenate dehydrogenase, at 1,213,317 to 1,214,651 _RS06575 MMJJ_RS06580: MMJJ_RS06580 - alanine dehydrogenase, at 1,214,947 to 1,216,068 _RS06580 MMJJ_RS06585: MMJJ_RS06585 - alanine racemase, at 1,216,124 to 1,217,245 _RS06585 MMJJ_RS06590: MMJJ_RS06590 - sodium:alanine symporter family protein, at 1,217,469 to 1,218,830 _RS06590 MMJJ_RS06595: MMJJ_RS06595 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA, at 1,219,010 to 1,220,305 _RS06595
Group Condition MMJJ_RS06575 MMJJ_RS06580 MMJJ_RS06585 MMJJ_RS06590 MMJJ_RS06595
iron Iron limitation (0.2x), formate media -3.7 +0.3 +0.5 +0.2 N.D.
attachment assay; attached sample McCas-formate glass attached -3.0 +0.2 -0.0 +0.2 N.D.
iron Iron limitation (0.2x), formate media -3.3 +0.3 +0.4 +0.2 N.D.
iron Iron limitation (0.2x), formate media -2.8 +0.3 +0.4 +0.2 N.D.
nitrogen source H2 N-free with 2mM Adenosine -1.9 +0.4 +0.2 -0.7 N.D.
stress Formate 100 mM hypophosphite -2.4 +0.1 +0.3 +0.3 N.D.
stress Formate 100 mM hypophosphite -2.0 +0.3 +0.6 +0.3 N.D.
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.8 +0.6 +0.6 -2.6 N.D.
stress McCas_formate_complete hypophosphite 62.5 mM +1.3 -0.3 +0.2 -1.5 N.D.
stress McCas_formate_complete Ticarcillin 12.5 mM +1.6 -0.8 -0.6 +0.4 N.D.
iron Iron excess (2.5x) , H2 media -1.0 +0.6 +0.7 +0.3 N.D.
iron Iron excess (2.5x) , H2 media -1.0 +0.7 +0.9 +0.2 N.D.
iron Iron excess (2.5x) , H2 media -0.8 +0.5 +0.8 +0.3 N.D.
nitrogen source Formate N-free with 2mM Adenosine -0.7 +1.1 +0.5 +0.1 N.D.
stress McCas_formate_complete Anhydrotetracycline 0.05 mM +1.9 -0.6 +0.1 -0.1 N.D.
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM +2.0 -0.4 -0.3 +0.1 N.D.
stress McCas_formate_complete phosphite 25 mM +2.4 -0.1 -0.4 -0.4 N.D.
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM +2.1 -0.4 -0.0 -0.1 N.D.
no stress control McCas_formate_complete no stress control +2.2 -0.3 +0.1 -0.0 N.D.
no stress control McCas_formate_complete no stress control +2.3 -0.0 -0.0 -0.2 N.D.
stress McCas_formate_complete Antimycin A 0.01 mM +2.0 -0.1 +0.1 +0.3 N.D.
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM +2.3 +0.0 +0.3 -0.3 N.D.
stress McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM +2.8 -0.2 -0.0 -0.2 N.D.
stress McCas_formate_complete silver chloride 0.125 mM +2.0 -0.1 +0.4 +0.1 N.D.
stress McCas_formate_complete Irgasan 0.0125 mM +2.5 -0.1 +0.2 -0.0 N.D.
stress McCas_formate_complete sodium bromide 125 mM +2.4 +0.2 +0.0 -0.1 N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +1.3 +0.4 +0.5 +0.3 N.D.
biofilm H2 pooled triplicate biofilm cells +1.5 +0.5 +0.5 +0.3 N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +2.2 +0.2 +0.3 +0.2 N.D.
planktonic H2 pooled triplicate planktonic cells +1.5 +0.9 +0.5 +0.3 N.D.
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