Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS06495 and MMJJ_RS06500 overlap by 4 nucleotidesMMJJ_RS06500 and MMJJ_RS06505 overlap by 4 nucleotidesMMJJ_RS06505 and MMJJ_RS06510 are separated by 110 nucleotidesMMJJ_RS06510 and MMJJ_RS06515 are separated by 42 nucleotides MMJJ_RS06495: MMJJ_RS06495 - hypothetical protein, at 1,198,515 to 1,198,805 _RS06495 MMJJ_RS06500: MMJJ_RS06500 - class I SAM-dependent methyltransferase, at 1,198,802 to 1,199,512 _RS06500 MMJJ_RS06505: MMJJ_RS06505 - prephenate dehydratase, at 1,199,509 to 1,200,315 _RS06505 MMJJ_RS06510: MMJJ_RS06510 - LL-diaminopimelate aminotransferase, at 1,200,426 to 1,201,676 _RS06510 MMJJ_RS06515: MMJJ_RS06515 - ribonuclease III, at 1,201,719 to 1,202,408 _RS06515
Group Condition MMJJ_RS06495 MMJJ_RS06500 MMJJ_RS06505 MMJJ_RS06510 MMJJ_RS06515
hydrogen no stress control H2 no stress control +0.1 +0.2 -0.1 -5.1 +0.2
attachment assay; attached sample McCas-formate glass attached +0.6 +0.2 -0.3 -4.5 +0.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.3 +0.5 -0.1 -4.6 +0.3
nitrogen source formate N-free with 10mM Ammonium chloride +0.1 +0.4 +0.3 -4.6 +0.2
nutrient t1 formate McAA nicotinic dropout +0.2 +0.3 +0.3 -4.7 +0.4
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.4 +0.3 -0.1 -4.4 +0.3
nutrient t1 formate vitamin complete +0.1 +0.4 +0.2 -4.6 +0.5
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.5 +0.3 -0.1 -4.3 +0.3
iron control, 1x iron with casaminos and vitamins, H2 media +0.7 +0.1 +0.2 -4.7 +0.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.5 +0.2 +0.2 -4.4 +0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.6 -0.0 +0.4 -4.4 +0.2
iron Iron limitation (0.2x), formate media -0.1 +0.6 +0.6 -4.5 +0.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.5 +0.2 +0.6 -4.5 +0.3
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +0.6 +0.5 +0.1 -4.3 +0.3
iron Iron limitation (0.2x), formate media +0.6 +0.3 +0.4 -4.5 +0.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.4 +0.3 +0.6 -4.5 +0.5
nutrient t1 formate McAA biotin dropout +0.4 +0.3 +0.3 -4.1 +0.4
nutrient t1 formate McAA nicotinic dropout +0.3 +0.5 +0.4 -4.1 +0.4
nutrient t1 formate McAA thiamine dropout +0.6 +0.5 +0.2 -4.2 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.4 +0.3 +0.4 -4.1 +0.4
nutrient t1 formate vitamin complete +0.1 +0.6 +0.4 -4.0 +0.5
iron Iron excess (2.5x) , H2 media +0.3 +0.3 +0.9 -4.6 +0.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.1 +0.9 +0.2 -4.2 +0.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.4 +0.8 +0.3 -4.2 +0.5
iron Iron limitation (0.2x), formate media +0.5 +0.5 +0.3 -4.1 +0.5
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.3 +1.0 +0.2 -4.3 +0.5
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.8 +0.2 +0.7 -4.1 +0.3
stress Formate 50 mM hypophosphite +0.3 +0.4 +0.8 -4.0 +0.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.5 +0.4 +0.8 -4.1 +0.5
iron Iron excess (2.5x) , H2 media +1.0 -0.0 +0.8 -3.5 +0.7
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