Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS06190 and MMJJ_RS06195 are separated by 83 nucleotidesMMJJ_RS06195 and MMJJ_RS06200 are separated by 142 nucleotidesMMJJ_RS06200 and MMJJ_RS06205 are separated by 32 nucleotidesMMJJ_RS06205 and MMJJ_RS06210 are separated by 22 nucleotides MMJJ_RS06190: MMJJ_RS06190 - 7-cyano-7-deazaguanine synthase, at 1,148,361 to 1,149,146 _RS06190 MMJJ_RS06195: MMJJ_RS06195 - glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit, at 1,149,230 to 1,150,330 _RS06195 MMJJ_RS06200: MMJJ_RS06200 - phosphoglycerate dehydrogenase, at 1,150,473 to 1,152,044 _RS06200 MMJJ_RS06205: MMJJ_RS06205 - EamA family transporter, at 1,152,077 to 1,152,490 _RS06205 MMJJ_RS06210: MMJJ_RS06210 - hypothetical protein, at 1,152,513 to 1,152,755 _RS06210
Group Condition MMJJ_RS06190 MMJJ_RS06195 MMJJ_RS06200 MMJJ_RS06205 MMJJ_RS06210
nitrogen source H2 N-free with 10mM D-glucosamine +0.1 N.D. -2.1 +0.2 -2.3
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM +0.4 N.D. -4.7 +0.5 -0.1
stress McCas_formate_complete selenate 31.25 mM +0.2 N.D. -2.8 +0.0 -1.3
stress McCas_formate_complete Nitrofurantoin 0.025 mM +0.1 N.D. -4.1 +0.8 -0.5
nitrogen source H2 N-free with 10mM D-glucosamine +0.1 N.D. -1.2 +0.1 -2.6
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM +0.6 N.D. -1.9 +0.2 -1.7
nutrient t1 formate McAA B12 dropout +0.2 N.D. -3.4 +0.3 +0.4
iron control, 1x iron with casaminos and vitamins, H2 media +0.4 N.D. -2.7 +0.6 -0.4
iron Iron excess (2.5x) , H2 media +0.4 N.D. -3.6 +0.7 +0.4
stress McCas_formate_complete Chlorpromazine 0.0166666666666667 mM +0.4 N.D. -3.9 +0.2 +1.4
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.3 N.D. -2.6 +0.2 +0.4
stress McCas_formate_complete phosphite 25 mM +0.3 N.D. -2.4 +0.4 +0.4
stress McCas_formate_complete Ticarcillin 12.5 mM +0.7 N.D. -2.4 +0.4 +0.1
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.6 N.D. -2.7 +0.6 +0.5
stress McCas_formate_complete Fluorophosphate 31.25 mM +0.5 N.D. -2.1 +0.7 -0.1
iron Iron excess (2.5x) , H2 media +0.4 N.D. -2.1 +0.5 +0.4
stress McCas_formate_complete deta nonoate 0.025 mM +0.4 N.D. -2.7 +0.6 +1.1
stress McCas_formate_complete silver chloride 0.125 mM +0.5 N.D. -2.1 +0.8 +0.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.4 N.D. -1.9 +0.6 +0.6
stress McCas_formate_complete 3-nitroxypropanol 0.00078125 mM +0.3 N.D. -1.9 +0.5 +0.9
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.3 N.D. -1.9 +0.2 +1.4
stress McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM +0.4 N.D. -2.1 +0.5 +1.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.4 N.D. -1.6 +0.5 +1.0
nitrogen source Formate N-free with 2mM Adenosine +0.2 N.D. -2.1 +1.3 +0.9
nitrogen source Formate N-free with 2mM Adenosine +0.5 N.D. -1.9 +1.7 +0.8
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.4 N.D. -1.1 +0.4 +2.1
nitrogen source H2 N-free with 2mM Adenosine +0.5 N.D. -1.6 +4.5 +0.5
nitrogen source H2 N-free with 2mM Adenosine +0.3 N.D. -0.2 +3.3 +0.5
nitrogen source Formate N-free with 2mM Adenosine +0.8 N.D. +0.8 +1.4 +1.1
nitrogen source H2 N-free with 2mM Adenosine +0.6 N.D. +0.9 +4.7 +0.0
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