Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05945 and MMJJ_RS05950 are separated by 129 nucleotidesMMJJ_RS05950 and MMJJ_RS05955 are separated by 9 nucleotidesMMJJ_RS05955 and MMJJ_RS05960 are separated by 186 nucleotidesMMJJ_RS05960 and MMJJ_RS05965 are separated by 10 nucleotides MMJJ_RS05945: MMJJ_RS05945 - methionine adenosyltransferase, at 1,105,663 to 1,106,880 _RS05945 MMJJ_RS05950: MMJJ_RS05950 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,107,010 to 1,108,212 _RS05950 MMJJ_RS05955: MMJJ_RS05955 - radical SAM protein, at 1,108,222 to 1,109,085 _RS05955 MMJJ_RS05960: MMJJ_RS05960 - DUF169 domain-containing protein, at 1,109,272 to 1,109,964 _RS05960 MMJJ_RS05965: MMJJ_RS05965 - MFS transporter, at 1,109,975 to 1,111,345 _RS05965
Group Condition MMJJ_RS05945 MMJJ_RS05950 MMJJ_RS05955 MMJJ_RS05960 MMJJ_RS05965
nutrient t1 formate McAA biotin dropout N.D. -4.6 +0.0 +0.1 +0.2
attachment assay; attached sample McCas-formate glass attached N.D. -4.3 -0.2 +0.1 +0.1
nutrient t1 formate vitamin complete N.D. -4.2 -0.0 +0.1 +0.0
attachment assay; attached sample McCas-formate glass attached N.D. -4.4 -0.0 +0.4 -0.0
iron Iron limitation (0.2x), H2 media N.D. -4.4 +0.2 -0.0 +0.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. -4.2 +0.1 +0.2 -0.0
nutrient t1 formate McAA B12 dropout N.D. -4.2 -0.0 +0.2 +0.1
nutrient t1 formate McAA biotin dropout N.D. -4.1 -0.0 +0.2 +0.1
nutrient t1 formate McAA lipolic dropout N.D. -4.3 +0.0 +0.3 +0.1
iron control, 1x iron with casaminos and vitamins, formate media N.D. -4.2 +0.0 +0.2 +0.2
iron Iron excess (2.5x) , formate media N.D. -4.2 +0.1 +0.2 +0.2
nutrient t1 formate McAA p-aminobenzoic dropout N.D. -4.3 +0.1 +0.3 +0.2
nutrient t1 formate McAA lipolic dropout N.D. -3.9 +0.0 +0.2 +0.1
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. -3.9 +0.3 +0.0 +0.1
nutrient t1 formate McAA pyroxidine HCl dropout N.D. -3.8 +0.1 +0.2 +0.2
iron Iron limitation (0.2x), formate media N.D. -3.8 +0.1 +0.2 +0.1
nutrient t1 formate McAA nicotinic dropout N.D. -3.8 +0.2 +0.1 +0.2
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. -3.8 +0.2 +0.3 +0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. -3.7 +0.2 +0.1 +0.3
nutrient t1 formate McAA biotin dropout N.D. -3.8 +0.1 +0.4 +0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. -3.8 +0.2 +0.1 +0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. -3.7 +0.2 +0.0 +0.3
nitrogen source H2 N-free with 10mM D-glucosamine N.D. -3.7 +0.3 +0.1 +0.2
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. -3.6 +0.2 +0.2 +0.2
iron Iron excess (2.5x) , H2 media N.D. -3.7 +0.2 +0.4 +0.1
iron Iron excess (2.5x) , H2 media N.D. -3.8 +0.4 +0.2 +0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. -3.5 +0.2 +0.2 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. -3.4 +0.4 +0.1 +0.4
iron Iron excess (2.5x) , H2 media N.D. -3.1 +0.4 +0.4 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE N.D. -3.1 +0.4 +0.2 +0.5
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