Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05925 and MMJJ_RS05930 are separated by 23 nucleotidesMMJJ_RS05930 and MMJJ_RS05935 are separated by 6 nucleotidesMMJJ_RS05935 and MMJJ_RS05940 are separated by 75 nucleotidesMMJJ_RS05940 and MMJJ_RS05945 are separated by 5 nucleotides MMJJ_RS05925: MMJJ_RS05925 - DUF2099 family protein, at 1,102,965 to 1,103,816 _RS05925 MMJJ_RS05930: MMJJ_RS05930 - cell division protein SepF, at 1,103,840 to 1,104,229 _RS05930 MMJJ_RS05935: MMJJ_RS05935 - Era-like GTP-binding protein, at 1,104,236 to 1,104,790 _RS05935 MMJJ_RS05940: MMJJ_RS05940 - HAD family hydrolase, at 1,104,866 to 1,105,657 _RS05940 MMJJ_RS05945: MMJJ_RS05945 - methionine adenosyltransferase, at 1,105,663 to 1,106,880 _RS05945
Group Condition MMJJ_RS05925 MMJJ_RS05930 MMJJ_RS05935 MMJJ_RS05940 MMJJ_RS05945
attachment assay; attached sample McCas-formate glass attached -0.5 -4.4 -5.0 +0.0 N.D.
iron Iron limitation (0.2x), H2 media +0.0 -4.3 -5.4 +0.0 N.D.
iron Iron limitation (0.2x), H2 media +0.0 -4.3 -5.3 +0.2 N.D.
nutrient t1 formate McAA B12 dropout +0.0 -4.5 -4.6 -0.1 N.D.
nutrient t1 formate McAA pantothenate dropout +0.0 -4.5 -4.7 -0.1 N.D.
iron Iron excess (2.5x) , H2 media -0.1 -4.0 -5.0 +0.4 N.D.
nitrogen source H2 N-free with 2g/L casaminos -0.1 -3.2 -5.3 +0.3 N.D.
nutrient t1 formate McAA biotin dropout +0.0 -4.5 -3.5 +0.1 N.D.
iron control, 1x iron with casaminos and vitamins, H2 media -0.1 -4.1 -3.8 +0.3 N.D.
nitrogen source H2 N-free with 10mM Ammonium chloride -0.1 -4.3 -3.3 +0.3 N.D.
iron Iron excess (2.5x) , H2 media +0.0 -4.1 -3.5 +0.1 N.D.
nutrient t1 formate McAA folic dropout +0.0 -3.6 -3.6 -0.1 N.D.
iron control, 1x iron with casaminos and vitamins, formate media -0.0 -4.5 -2.7 +0.0 N.D.
nutrient t1 formate McAA nicotinic dropout +0.1 -3.3 -4.0 +0.0 N.D.
no stress control McCas_formate_complete no stress control -0.0 -3.0 -3.7 -0.4 N.D.
nitrogen source H2 N-free with 10mM D-glucosamine -0.2 -3.6 -3.3 +0.1 N.D.
nitrogen source H2 N-free with 2g/L casaminos -0.1 -4.1 -2.8 +0.1 N.D.
formate no stress control Formate no stress control -0.1 -5.4 -1.5 +0.1 N.D.
iron control, 1x iron with casaminos and vitamins, H2 media -0.1 -3.5 -3.5 +0.2 N.D.
stress McCas_formate_complete Rifampicin 0.25 mM +0.0 -2.8 -3.5 -0.5 N.D.
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM -0.2 -3.0 -3.6 +0.1 N.D.
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.1 -3.6 -3.2 +0.2 N.D.
nutrient t1 formate McAA thiamine dropout +0.1 -3.0 -3.7 +0.1 N.D.
nutrient t1 formate McAA thiamine dropout +0.1 -3.2 -3.6 +0.1 N.D.
nutrient t1 formate McAA folic dropout +0.1 -3.7 -3.0 +0.0 N.D.
nitrogen source H2 N-free with 10mM Ammonium chloride +0.1 -3.5 -3.2 +0.1 N.D.
stress Formate 100 mM hypophosphite +0.1 -4.3 -2.4 +0.1 N.D.
iron control, 1x iron with casaminos and vitamins, H2 media -0.1 -3.4 -3.1 +0.2 N.D.
iron Iron excess (2.5x) , H2 media -0.1 -4.0 -2.4 +0.3 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.5 -2.4 -3.7 +0.2 N.D.
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