Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05800 and MMJJ_RS05805 are separated by 50 nucleotidesMMJJ_RS05805 and MMJJ_RS05810 are separated by 29 nucleotidesMMJJ_RS05810 and MMJJ_RS05815 overlap by 4 nucleotidesMMJJ_RS05815 and MMJJ_RS05820 overlap by 11 nucleotides MMJJ_RS05800: MMJJ_RS05800 - coenzyme F420-0:L-glutamate ligase, at 1,080,938 to 1,081,834 _RS05800 MMJJ_RS05805: MMJJ_RS05805 - aconitase X catalytic domain-containing protein, at 1,081,885 to 1,083,090 _RS05805 MMJJ_RS05810: MMJJ_RS05810 - precorrin-4 C(11)-methyltransferase, at 1,083,120 to 1,083,884 _RS05810 MMJJ_RS05815: MMJJ_RS05815 - hypothetical protein, at 1,083,881 to 1,084,078 _RS05815 MMJJ_RS05820: MMJJ_RS05820 - DUF2304 domain-containing protein, at 1,084,068 to 1,084,427 _RS05820
Group Condition MMJJ_RS05800 MMJJ_RS05805 MMJJ_RS05810 MMJJ_RS05815 MMJJ_RS05820
nitrogen source Formate N-free with 2mM Adenosine -2.6 N.D. -2.9 -0.3 -1.1
stress McCas_formate_complete Chlorpromazine 0.0166666666666667 mM -0.3 N.D. -0.5 -1.2 -3.4
nitrogen source Formate N-free with 2mM Adenosine -1.7 N.D. -2.8 +0.3 -1.1
iron control, 1x iron with casaminos and vitamins, H2 media -0.5 N.D. -4.6 -0.0 -0.2
nutrient t1 formate McAA B12 dropout -0.9 N.D. -3.3 -0.2 -0.9
nitrogen source H2 N-free with 2mM Adenosine -1.4 N.D. -2.5 +0.0 -1.3
iron Iron excess (2.5x) , H2 media -0.4 N.D. -4.2 -0.0 -0.6
nitrogen source Formate N-free with 2mM Adenosine -2.1 N.D. -2.4 +0.1 -0.7
nutrient t1 formate McAA B12 dropout -1.0 N.D. -3.0 -0.1 -0.9
iron control, 1x iron with casaminos and vitamins, H2 media -0.5 N.D. -3.8 -0.2 -0.3
nitrogen source H2 N-free with 10mM Ammonium chloride -0.3 N.D. -3.8 +0.0 -0.8
nitrogen source H2 N-free with 10mM D-glucosamine -0.8 N.D. -3.0 +0.3 -1.1
nitrogen source H2 N-free with 10mM Ammonium chloride -0.3 N.D. -3.6 +0.0 -0.8
nitrogen source H2 N-free with 2g/L casaminos -0.6 N.D. -3.7 +0.0 -0.3
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.8 N.D. -3.5 -0.0 -0.1
nitrogen source H2 N-free with 2g/L casaminos -0.3 N.D. -3.8 +0.2 -0.7
iron Iron excess (2.5x) , H2 media -0.5 N.D. -3.3 +0.1 -0.7
iron Iron limitation (0.2x), H2 media -0.6 N.D. -3.3 +0.2 -0.7
iron Iron excess (2.5x) , H2 media -0.0 N.D. -4.1 +0.2 -0.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.6 N.D. -3.7 +0.2 -0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -1.3 N.D. -3.3 +0.6 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.8 N.D. -3.6 -0.1 +0.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.4 N.D. -3.9 -0.0 +0.3
planktonic H2 pooled triplicate planktonic cells -1.1 N.D. -3.5 +0.4 +0.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -1.0 N.D. -3.1 +0.5 -0.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE -0.8 N.D. -1.9 +0.7 -1.6
stress McCas_formate_complete deta nonoate 0.025 mM -1.0 N.D. -2.7 +0.5 -0.4
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.3 N.D. -3.1 +0.4 +1.1
biofilm H2 pooled triplicate biofilm cells -0.2 N.D. -3.1 +0.7 +1.0
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.9 N.D. -3.3 +0.3 +1.1
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