Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05760 and MMJJ_RS05765 overlap by 11 nucleotidesMMJJ_RS05765 and MMJJ_RS05770 are separated by 27 nucleotidesMMJJ_RS05770 and MMJJ_RS05775 overlap by 11 nucleotidesMMJJ_RS05775 and MMJJ_RS05780 are separated by 10 nucleotides MMJJ_RS05760: MMJJ_RS05760 - flagellar protein F, at 1,074,570 to 1,074,977 _RS05760 MMJJ_RS05765: MMJJ_RS05765 - flagellar protein E, at 1,074,967 to 1,075,374 _RS05765 MMJJ_RS05770: MMJJ_RS05770 - flagellar protein D, at 1,075,402 to 1,076,370 _RS05770 MMJJ_RS05775: MMJJ_RS05775 - flagellar protein C, at 1,076,360 to 1,076,920 _RS05775 MMJJ_RS05780: MMJJ_RS05780 - flagellin, at 1,076,931 to 1,077,563 _RS05780
Group Condition MMJJ_RS05760 MMJJ_RS05765 MMJJ_RS05770 MMJJ_RS05775 MMJJ_RS05780
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.0 -3.0 -4.9 N.D. -3.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.1 -3.0 -4.9 N.D. -2.8
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.3 -2.9 -4.8 N.D. -2.8
stress McCas_formate_complete selenate 31.25 mM +0.4 -2.8 -4.2 N.D. -3.5
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 -2.9 -4.0 N.D. -3.0
attachment assay; attached sample McCas-formate glass attached -0.5 -2.9 -2.2 N.D. -4.0
stress McCas_formate_complete deta nonoate 0.025 mM +0.3 -3.0 -4.1 N.D. -2.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -2.2 -4.3 N.D. -2.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 -2.0 -4.6 N.D. -2.5
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.9 -3.1 -3.3 N.D. -3.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.1 -3.0 -4.2 N.D. -1.8
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -2.9 -4.0 N.D. -2.0
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 -2.1 -4.2 N.D. -2.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +1.0 -2.6 -3.8 N.D. -3.5
iron Iron excess (2.5x) , H2 media -0.4 -3.5 -2.4 N.D. -2.4
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.8 -3.1 -3.4 N.D. -3.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.1 -3.0 -2.4 N.D. -3.3
attachment assay; attached sample McCas-formate glass attached -0.7 -4.5 -0.2 N.D. -3.1
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.1 -1.9 -4.4 N.D. -2.3
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM +0.8 -3.8 -1.6 N.D. -3.8
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.8 -3.1 -3.3 N.D. -2.6
stress McCas_formate_complete sulfate 125 mM +0.3 -3.5 -4.0 N.D. -1.0
stress McCas_formate_complete Rifampicin 0.25 mM +0.3 -1.5 -4.5 N.D. -2.6
stress McCas_formate_complete deta nonoate 0.025 mM +0.8 -2.6 -4.0 N.D. -2.3
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +1.0 -2.2 -2.8 N.D. -3.5
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM +0.8 -3.5 -2.8 N.D. -2.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +1.1 -3.0 -2.8 N.D. -2.3
stress McCas_formate_complete 3-nitroxypropanol 0.00078125 mM +1.0 -1.8 -4.1 N.D. -2.1
no stress control McCas_formate_complete no stress control +1.3 -2.7 -3.7 N.D. -1.7
nitrogen source N2 fixation, H2 media -0.2 +8.2 +0.3 N.D. +0.8
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