Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05720 and MMJJ_RS05725 are separated by 110 nucleotidesMMJJ_RS05725 and MMJJ_RS05730 are separated by 3 nucleotidesMMJJ_RS05730 and MMJJ_RS05735 are separated by 73 nucleotidesMMJJ_RS05735 and MMJJ_RS05740 are separated by 13 nucleotides MMJJ_RS05720: MMJJ_RS05720 - glutamine--fructose-6-phosphate transaminase (isomerizing), at 1,065,936 to 1,067,735 _RS05720 MMJJ_RS05725: MMJJ_RS05725 - UPF0179 family protein, at 1,067,846 to 1,068,283 _RS05725 MMJJ_RS05730: MMJJ_RS05730 - TIM barrel protein, at 1,068,287 to 1,069,108 _RS05730 MMJJ_RS05735: MMJJ_RS05735 - UbiA family prenyltransferase, at 1,069,182 to 1,070,018 _RS05735 MMJJ_RS05740: MMJJ_RS05740 - archaellar assembly protein FlaJ, at 1,070,032 to 1,071,708 _RS05740
Group Condition MMJJ_RS05720 MMJJ_RS05725 MMJJ_RS05730 MMJJ_RS05735 MMJJ_RS05740
attachment assay; attached sample McCas-formate glass attached -5.0 -0.4 +0.2 N.D. -0.7
planktonic H2 pooled triplicate planktonic cells -2.5 -2.5 +0.0 N.D. -0.5
nitrogen source H2 N-free with 10mM D-glucosamine -3.2 -2.6 +0.4 N.D. -0.2
stress Formate 50 mM hypophosphite -4.0 -1.4 +0.1 N.D. +0.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -3.8 -1.2 +0.2 N.D. -0.3
nitrogen source H2 N-free with 10mM D-glucosamine -3.3 -1.6 +0.3 N.D. -0.3
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM -2.4 -1.8 -0.7 N.D. +0.1
iron control, 1x iron with casaminos and vitamins, H2 media -3.7 -1.1 +0.2 N.D. -0.1
iron control, 1x iron with casaminos and vitamins, H2 media -3.8 -1.1 +0.2 N.D. -0.1
nitrogen source H2 N-free with 10mM Ammonium chloride -3.2 -1.2 +0.3 N.D. -0.4
biofilm H2 pooled triplicate biofilm cells -2.5 -1.8 +0.3 N.D. -0.7
iron Iron excess (2.5x) , H2 media -3.4 -1.2 +0.4 N.D. -0.3
stress McCas_formate_complete Spiramycin 0.1 mM -3.6 -1.5 -0.2 N.D. +1.0
stress McCas_formate_complete silver chloride 0.125 mM -3.6 -1.1 -0.5 N.D. +0.9
iron Iron excess (2.5x) , H2 media -3.0 -1.3 +0.5 N.D. -0.4
stress McCas_formate_complete sulfate 125 mM -2.8 -1.5 -0.2 N.D. +0.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -2.8 -1.5 -0.3 N.D. +0.9
stress McCas_formate_complete Ticarcillin 12.5 mM -3.1 -0.6 -0.6 N.D. +0.8
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -2.5 -1.5 -0.3 N.D. +0.9
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -2.5 -1.6 -0.3 N.D. +1.0
nitrogen source Formate N-free with 2mM Adenosine -3.2 +1.1 +0.1 N.D. -1.3
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM -2.7 -1.1 -0.3 N.D. +1.0
stress McCas_formate_complete silver chloride 0.125 mM -2.5 -1.0 -0.6 N.D. +1.0
no stress control McCas_formate_complete no stress control -3.5 -0.4 -0.4 N.D. +1.3
stress McCas_formate_complete phosphite 25 mM -2.6 -0.9 -0.7 N.D. +1.1
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -2.8 -1.3 +0.1 N.D. +1.0
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -3.0 -1.2 +0.1 N.D. +1.1
stress McCas_formate_complete Fluorophosphate 31.25 mM -2.8 -1.1 -0.4 N.D. +1.4
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -2.9 -1.1 +0.1 N.D. +1.1
no stress control McCas_formate_complete no stress control -2.2 -1.3 -0.5 N.D. +1.4
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