Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05700 and MMJJ_RS05705 are separated by 0 nucleotidesMMJJ_RS05705 and MMJJ_RS05710 are separated by 132 nucleotidesMMJJ_RS05710 and MMJJ_RS05715 are separated by 48 nucleotidesMMJJ_RS05715 and MMJJ_RS05720 overlap by 14 nucleotides MMJJ_RS05700: MMJJ_RS05700 - metallophosphoesterase, at 1,061,753 to 1,062,436 _RS05700 MMJJ_RS05705: MMJJ_RS05705 - hypothetical protein, at 1,062,437 to 1,063,078 _RS05705 MMJJ_RS05710: MMJJ_RS05710 - single-stranded-DNA-specific exonuclease RecJ, at 1,063,211 to 1,064,614 _RS05710 MMJJ_RS05715: MMJJ_RS05715 - phosphoadenosine phosphosulfate reductase family protein, at 1,064,663 to 1,065,949 _RS05715 MMJJ_RS05720: MMJJ_RS05720 - glutamine--fructose-6-phosphate transaminase (isomerizing), at 1,065,936 to 1,067,735 _RS05720
Group Condition MMJJ_RS05700 MMJJ_RS05705 MMJJ_RS05710 MMJJ_RS05715 MMJJ_RS05720
attachment assay; attached sample McCas-formate glass attached +0.1 +0.2 -2.7 -0.1 -5.0
no stress control McCas_formate_complete no stress control -0.0 +0.0 -2.7 -0.1 -3.5
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM -0.9 +0.4 -2.3 -0.2 -2.7
stress Formate 50 mM hypophosphite +0.1 +0.0 -1.8 -0.0 -4.0
stress McCas_formate_complete selenate 31.25 mM -0.4 +0.0 -1.3 +0.1 -4.0
stress McCas_formate_complete Ticarcillin 12.5 mM -0.5 +0.5 -1.8 -0.2 -3.1
stress McCas_formate_complete silver chloride 0.125 mM -0.3 +0.1 -2.6 +0.0 -2.5
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.2 -0.1 -2.5 -0.1 -2.8
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM +0.2 +0.4 -2.9 +0.2 -3.1
iron control, 1x iron with casaminos and vitamins, H2 media +0.2 +0.2 -1.8 +0.0 -3.8
iron Iron excess (2.5x) , H2 media +0.3 +0.3 -2.1 -0.1 -3.4
nitrogen source H2 N-free with 10mM Ammonium chloride +0.3 +0.1 -2.6 +0.0 -2.9
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM -0.3 +0.1 -1.7 -0.2 -3.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.5 +0.1 -1.8 +0.0 -3.8
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.6 +0.2 -2.1 +0.1 -3.6
nitrogen source H2 N-free with 10mM D-glucosamine +0.3 +0.1 -2.0 +0.1 -3.3
nitrogen source H2 N-free with 10mM D-glucosamine +0.3 +0.1 -2.2 +0.2 -3.2
no stress control McCas_formate_complete no stress control +0.4 +0.3 -2.0 -0.1 -3.3
nutrient t1 formate McAA p-aminobenzoic dropout +0.2 +0.0 -1.3 +0.2 -3.7
stress McCas_formate_complete Spiramycin 0.1 mM +0.1 +0.1 -1.3 +0.2 -3.6
nitrogen source H2 N-free with 10mM Ammonium chloride +0.4 +0.2 -1.9 +0.0 -3.2
nitrogen source H2 N-free with 10mM Ammonium chloride +0.4 +0.2 -2.3 +0.0 -2.8
iron control, 1x iron with casaminos and vitamins, H2 media +0.4 +0.1 -1.4 +0.2 -3.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.6 +0.1 -1.8 +0.1 -3.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.4 +0.2 -2.0 +0.1 -2.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.7 +0.0 -2.1 -0.0 -2.5
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.7 +0.1 -1.6 -0.1 -3.0
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.2 +0.4 -1.8 +0.1 -2.8
iron Iron excess (2.5x) , H2 media +0.7 +0.3 -1.4 +0.2 -3.0
nitrogen source Formate N-free with 2mM Adenosine +0.7 +0.1 -1.4 +0.7 -3.2
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