Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05635 and MMJJ_RS05645 are separated by 239 nucleotidesMMJJ_RS05645 and MMJJ_RS05650 are separated by 29 nucleotidesMMJJ_RS05650 and MMJJ_RS05655 are separated by 209 nucleotidesMMJJ_RS05655 and MMJJ_RS05660 are separated by 1 nucleotides MMJJ_RS05635: MMJJ_RS05635 - Ni/Fe hydrogenase subunit alpha, at 1,051,431 to 1,052,687 _RS05635 MMJJ_RS05645: MMJJ_RS05645 - 4Fe-4S binding protein, at 1,052,927 to 1,054,114 _RS05645 MMJJ_RS05650: MMJJ_RS05650 - formylmethanofuran dehydrogenase subunit B, at 1,054,144 to 1,055,448 _RS05650 MMJJ_RS05655: MMJJ_RS05655 - HisA/HisF family protein, at 1,055,658 to 1,056,326 _RS05655 MMJJ_RS05660: MMJJ_RS05660 - sulfopyruvate decarboxylase subunit beta, at 1,056,328 to 1,056,885 _RS05660
Group Condition MMJJ_RS05635 MMJJ_RS05645 MMJJ_RS05650 MMJJ_RS05655 MMJJ_RS05660
formate no stress control Formate no stress control N.D. -1.6 -5.3 -2.5 -0.3
hydrogen no stress control H2 no stress control N.D. -1.6 -4.6 -3.1 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. -1.2 -3.7 -3.0 -1.4
iron Iron limitation (0.2x), H2 media N.D. -1.4 -4.6 -3.0 -0.2
hydrogen no stress control H2 no stress control N.D. -1.5 -4.7 -2.8 -0.2
nutrient t1 formate McAA folic dropout N.D. -1.6 -4.9 -2.8 +0.2
iron Iron excess (2.5x) , H2 media N.D. -2.3 -4.0 -2.3 -0.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. -1.4 -4.0 -3.3 -0.1
nutrient t1 formate vitamin complete N.D. -1.4 -4.8 -2.5 +0.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. -1.4 -3.7 -3.2 -0.4
nutrient t1 formate McAA B12 dropout N.D. -1.5 -4.4 -3.1 +0.3
iron control, 1x iron with casaminos and vitamins, formate media N.D. -1.3 -4.5 -3.1 +0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. -1.0 -3.9 -3.2 -0.5
nutrient t1 formate McAA pyroxidine HCl dropout N.D. -1.4 -4.8 -2.3 +0.0
iron Iron limitation (0.2x), formate media N.D. -1.4 -4.7 -2.5 +0.2
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. -1.4 -4.5 -2.3 -0.3
nutrient t1 formate McAA thiamine dropout N.D. -1.3 -4.7 -2.6 +0.2
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. -1.4 -4.4 -2.1 -0.5
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. -1.3 -4.1 -2.9 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. -1.3 -4.0 -2.9 -0.2
iron Iron excess (2.5x) , formate media N.D. -1.3 -4.2 -2.7 +0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. -0.8 -4.0 -3.2 -0.2
nutrient t1 formate McAA pantothenate dropout N.D. -0.8 -4.8 -2.8 +0.3
nutrient t1 formate McAA pyroxidine HCl dropout N.D. -1.0 -4.4 -2.7 +0.3
nutrient t1 formate McAA B12 dropout N.D. -1.3 -3.5 -3.3 +0.3
nutrient t1 formate McAA p-aminobenzoic dropout N.D. -1.6 -3.6 -2.8 +0.3
biofilm H2 pooled triplicate biofilm cells N.D. -1.3 -3.9 -2.8 +0.6
planktonic H2 pooled triplicate planktonic cells N.D. -1.1 -4.0 -3.2 +0.9
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. -1.1 -3.6 -3.1 +0.7
nitrogen source Formate N-free with 2mM Adenosine N.D. -0.2 -3.8 -3.5 +1.0
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