Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05485 and MMJJ_RS05490 are separated by 73 nucleotidesMMJJ_RS05490 and MMJJ_RS05495 are separated by 31 nucleotidesMMJJ_RS05495 and MMJJ_RS05500 are separated by 11 nucleotidesMMJJ_RS05500 and MMJJ_RS05505 are separated by 194 nucleotides MMJJ_RS05485: MMJJ_RS05485 - 5S ribosomal RNA, at 1,025,494 to 1,025,608 _RS05485 MMJJ_RS05490: MMJJ_RS05490 - imidazole glycerol phosphate synthase subunit HisF, at 1,025,682 to 1,026,500 _RS05490 MMJJ_RS05495: MMJJ_RS05495 - MinD/ParA family protein, at 1,026,532 to 1,027,320 _RS05495 MMJJ_RS05500: MMJJ_RS05500 - hypothetical protein, at 1,027,332 to 1,029,125 _RS05500 MMJJ_RS05505: MMJJ_RS05505 - hypothetical protein, at 1,029,320 to 1,031,974 _RS05505
Group Condition MMJJ_RS05485 MMJJ_RS05490 MMJJ_RS05495 MMJJ_RS05500 MMJJ_RS05505
hydrogen no stress control H2 no stress control N.D. -4.9 +0.1 +0.1 +0.2
nitrogen source H2 N-free with 10mM D-glucosamine N.D. -3.4 -0.7 -0.3 +0.2
nutrient t1 formate McAA nicotinic dropout N.D. -4.3 -0.1 +0.2 +0.1
nutrient t1 formate McAA folic dropout N.D. -4.5 +0.2 +0.2 -0.0
nutrient t1 formate McAA lipolic dropout N.D. -4.4 +0.1 +0.2 +0.2
stress Formate 50 mM hypophosphite N.D. -4.1 -0.3 +0.1 +0.3
nutrient t1 formate vitamin complete N.D. -4.3 +0.0 +0.2 +0.1
nutrient t1 formate McAA pantothenate dropout N.D. -4.4 +0.0 +0.2 +0.1
nutrient t1 formate McAA pantothenate dropout N.D. -4.3 -0.1 +0.3 +0.1
nutrient t1 formate McAA riboflavin dropout N.D. -4.3 -0.0 +0.3 +0.1
nutrient t1 formate McAA biotin dropout N.D. -4.2 -0.0 +0.2 +0.1
attachment assay; attached sample McCas-formate glass attached N.D. -3.7 +0.1 +0.1 -0.4
stress Formate 50 mM hypophosphite N.D. -3.7 -0.4 +0.1 +0.3
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. -4.1 +0.2 +0.1 +0.3
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. -3.9 +0.0 +0.2 +0.3
nutrient t1 formate McAA folic dropout N.D. -3.9 +0.1 +0.2 +0.2
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. -4.0 +0.3 +0.1 +0.3
nutrient t1 formate McAA nicotinic dropout N.D. -3.9 +0.3 +0.3 +0.1
iron control, 1x iron with casaminos and vitamins, H2 media N.D. -3.9 +0.3 +0.2 +0.3
iron Iron excess (2.5x) , H2 media N.D. -3.8 +0.3 +0.3 +0.2
nitrogen source H2 N-free with 2g/L casaminos N.D. -3.6 +0.3 +0.2 +0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. -3.2 +0.4 +0.3 +0.7
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE N.D. -3.2 +0.2 +0.3 +0.9
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. -3.3 +0.4 +0.4 +0.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. -3.1 +0.4 +0.3 +0.5
stress McCas_formate_complete Chlorite 0.3125 mM N.D. -3.0 +0.3 +0.3 +0.8
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. -3.0 +0.5 +0.3 +0.8
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE N.D. -2.9 +0.4 +0.3 +1.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE N.D. -2.6 +0.4 +0.3 +1.0
nitrogen source Formate N-free with 2mM Adenosine N.D. -3.3 +1.4 +0.8 +0.6
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