Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05480 and MMJJ_RS05485 are separated by 56 nucleotidesMMJJ_RS05485 and MMJJ_RS05490 are separated by 73 nucleotidesMMJJ_RS05490 and MMJJ_RS05495 are separated by 31 nucleotidesMMJJ_RS05495 and MMJJ_RS05500 are separated by 11 nucleotides MMJJ_RS05480: MMJJ_RS05480 - 23S ribosomal RNA, at 1,022,470 to 1,025,437 _RS05480 MMJJ_RS05485: MMJJ_RS05485 - 5S ribosomal RNA, at 1,025,494 to 1,025,608 _RS05485 MMJJ_RS05490: MMJJ_RS05490 - imidazole glycerol phosphate synthase subunit HisF, at 1,025,682 to 1,026,500 _RS05490 MMJJ_RS05495: MMJJ_RS05495 - MinD/ParA family protein, at 1,026,532 to 1,027,320 _RS05495 MMJJ_RS05500: MMJJ_RS05500 - hypothetical protein, at 1,027,332 to 1,029,125 _RS05500
Group Condition MMJJ_RS05480 MMJJ_RS05485 MMJJ_RS05490 MMJJ_RS05495 MMJJ_RS05500
hydrogen no stress control H2 no stress control N.D. N.D. -4.9 +0.1 +0.1
nitrogen source H2 N-free with 10mM D-glucosamine N.D. N.D. -3.4 -0.7 -0.3
stress Formate 50 mM hypophosphite N.D. N.D. -4.1 -0.3 +0.1
nutrient t1 formate McAA nicotinic dropout N.D. N.D. -4.3 -0.1 +0.2
nutrient t1 formate McAA lipolic dropout N.D. N.D. -4.4 +0.1 +0.2
nutrient t1 formate McAA pantothenate dropout N.D. N.D. -4.4 +0.0 +0.2
nutrient t1 formate McAA folic dropout N.D. N.D. -4.5 +0.2 +0.2
nutrient t1 formate vitamin complete N.D. N.D. -4.3 +0.0 +0.2
nutrient t1 formate McAA pantothenate dropout N.D. N.D. -4.3 -0.1 +0.3
nutrient t1 formate McAA riboflavin dropout N.D. N.D. -4.3 -0.0 +0.3
nutrient t1 formate McAA biotin dropout N.D. N.D. -4.2 -0.0 +0.2
stress Formate 50 mM hypophosphite N.D. N.D. -3.7 -0.4 +0.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. N.D. -3.9 +0.0 +0.2
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. N.D. -4.1 +0.2 +0.1
formate no stress control Formate no stress control N.D. N.D. -3.7 -0.2 +0.1
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. N.D. -4.0 +0.3 +0.1
iron Iron excess (2.5x) , H2 media N.D. N.D. -3.8 +0.2 +0.0
nutrient t1 formate McAA folic dropout N.D. N.D. -3.9 +0.1 +0.2
attachment assay; attached sample McCas-formate glass attached N.D. N.D. -3.7 +0.1 +0.1
nutrient t1 formate McAA pantothenate dropout N.D. N.D. -3.8 +0.2 +0.2
iron Iron limitation (0.2x), formate media N.D. N.D. -3.7 +0.0 +0.2
iron control, 1x iron with casaminos and vitamins, H2 media N.D. N.D. -3.9 +0.3 +0.2
nutrient t1 formate McAA nicotinic dropout N.D. N.D. -3.9 +0.3 +0.3
iron Iron excess (2.5x) , H2 media N.D. N.D. -3.8 +0.3 +0.3
nitrogen source H2 N-free with 2g/L casaminos N.D. N.D. -3.6 +0.3 +0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. N.D. -3.4 +0.2 +0.2
nutrient t1 formate McAA B12 dropout N.D. N.D. -3.4 +0.3 +0.3
nutrient t1 formate McAA thiamine dropout N.D. N.D. -3.3 +0.3 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. N.D. -3.3 +0.4 +0.4
nitrogen source Formate N-free with 2mM Adenosine N.D. N.D. -3.3 +1.4 +0.8
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