Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS05330 and MMJJ_RS05335 are separated by 67 nucleotidesMMJJ_RS05335 and MMJJ_RS05340 are separated by 55 nucleotidesMMJJ_RS05340 and MMJJ_RS05345 are separated by 179 nucleotidesMMJJ_RS05345 and MMJJ_RS05350 overlap by 4 nucleotides MMJJ_RS05330: MMJJ_RS05330 - DNA polymerase II large subunit, at 988,990 to 992,385 _RS05330 MMJJ_RS05335: MMJJ_RS05335 - heavy metal-binding domain-containing protein, at 992,453 to 992,725 _RS05335 MMJJ_RS05340: MMJJ_RS05340 - AAA family ATPase, at 992,781 to 993,896 _RS05340 MMJJ_RS05345: MMJJ_RS05345 - ATP-dependent Clp protease proteolytic subunit, at 994,076 to 994,900 _RS05345 MMJJ_RS05350: MMJJ_RS05350 - DUF192 domain-containing protein, at 994,897 to 995,232 _RS05350
Group Condition MMJJ_RS05330 MMJJ_RS05335 MMJJ_RS05340 MMJJ_RS05345 MMJJ_RS05350
stress Formate 100 mM hypophosphite -3.4 +0.1 +0.2 -0.7 -0.5
nutrient t1 formate vitamin complete -3.9 +0.1 +0.0 -0.2 -0.0
nutrient t1 formate McAA p-aminobenzoic dropout -3.9 +0.0 +0.1 -0.0 -0.0
nutrient t1 formate McAA pantothenate dropout -3.5 -0.1 +0.2 -0.2 +0.1
iron Iron excess (2.5x) , formate media -3.9 +0.1 +0.1 -0.0 +0.2
nutrient t1 formate McAA lipolic dropout -3.6 +0.1 +0.2 -0.1 +0.1
biofilm Formate pooled triplicate biofilm cells -3.8 +0.2 -0.1 +0.1 +0.1
nitrogen source formate N-free with 10mM Ammonium chloride -4.0 +0.2 +0.1 -0.1 +0.3
nitrogen source H2 N-free with 10mM Ammonium chloride -3.8 +0.1 +0.2 -0.1 +0.2
iron Iron limitation (0.2x), H2 media -3.8 +0.0 +0.1 -0.1 +0.5
nutrient t1 formate vitamin complete -4.0 +0.4 +0.1 -0.0 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -2.5 +0.6 -0.4 -0.8 -0.1
nutrient t1 formate vitamin complete -3.6 +0.1 +0.2 -0.1 +0.3
nutrient t1 formate McAA pyroxidine HCl dropout -3.5 +0.1 +0.2 -0.1 +0.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -2.8 +0.6 -0.4 -0.7 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -2.8 +0.5 -0.4 -0.3 +0.2
nitrogen source H2 N-free with 2g/L casaminos -3.7 +0.2 +0.1 -0.1 +0.6
iron control, 1x iron with casaminos and vitamins, H2 media -3.5 +0.1 +0.1 -0.0 +0.4
iron control, 1x iron with casaminos and vitamins, H2 media -3.6 +0.2 +0.0 +0.1 +0.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -2.2 +0.6 -0.7 -0.7 +0.2
nitrogen source H2 N-free with 2g/L casaminos -3.7 +0.3 +0.1 +0.0 +0.5
iron Iron excess (2.5x) , H2 media -3.6 +0.1 +0.1 +0.1 +0.5
nitrogen source H2 N-free with 10mM Ammonium chloride -3.2 +0.2 +0.2 -0.2 +0.4
nitrogen source H2 N-free with 10mM Ammonium chloride -3.6 +0.3 +0.3 -0.1 +0.5
nitrogen source H2 N-free with 2g/L casaminos -3.5 +0.2 +0.2 +0.0 +0.6
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -3.3 +0.3 +0.2 -0.1 +0.3
iron Iron excess (2.5x) , H2 media -3.5 +0.3 +0.1 +0.1 +0.5
iron Iron excess (2.5x) , H2 media -3.4 +0.4 +0.1 +0.2 +0.5
biofilm H2 pooled triplicate biofilm cells -3.2 +0.5 +0.2 +0.4 +0.3
planktonic H2 pooled triplicate planktonic cells -3.1 +0.5 +0.4 +0.4 +0.7
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