Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS04965 and MMJJ_RS04970 are separated by 100 nucleotidesMMJJ_RS04970 and MMJJ_RS04975 are separated by 22 nucleotidesMMJJ_RS04975 and MMJJ_RS04980 are separated by 15 nucleotidesMMJJ_RS04980 and MMJJ_RS04985 are separated by 4 nucleotides MMJJ_RS04965: MMJJ_RS04965 - RNA polymerase Rpb4 family protein, at 922,804 to 923,127 _RS04965 MMJJ_RS04970: MMJJ_RS04970 - DUF655 domain-containing protein, at 923,228 to 923,812 _RS04970 MMJJ_RS04975: MMJJ_RS04975 - glycosyltransferase family 4 protein, at 923,835 to 924,902 _RS04975 MMJJ_RS04980: MMJJ_RS04980 - bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase, at 924,918 to 925,523 _RS04980 MMJJ_RS04985: MMJJ_RS04985 - glutamyl-tRNA reductase, at 925,528 to 926,676 _RS04985
Group Condition MMJJ_RS04965 MMJJ_RS04970 MMJJ_RS04975 MMJJ_RS04980 MMJJ_RS04985
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -3.1 -3.8 -3.1 -4.6 -2.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -1.7 -4.3 -3.7 -4.4 -1.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.7 -4.5 -3.8 -4.5 -1.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.5 -4.3 -3.6 -4.8 -1.5
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM -2.4 -4.8 -3.3 -1.8 -1.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.3 -4.4 -3.7 -3.6 -1.9
hydrogen no stress control H2 no stress control -1.2 -4.3 -2.1 -3.0 -2.4
formate no stress control Formate no stress control -0.4 -4.7 -4.0 -2.3 -1.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.0 -4.1 -2.9 -3.7 -1.9
stress Formate 50 mM hypophosphite -0.2 -2.9 -5.4 -2.5 -1.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.5 -3.5 -3.8 -3.2 -1.5
stress McCas_formate_complete deta nonoate 0.025 mM -1.7 -3.2 -4.4 -1.9 -1.2
stress Formate 50 mM hypophosphite +0.1 -4.0 -5.4 -1.7 -1.1
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -1.0 -3.4 -2.5 -2.9 -2.2
nitrogen source H2 N-free with 10mM D-glucosamine -0.3 -3.7 -3.3 -2.4 -2.3
formate no stress control Formate no stress control -0.3 -3.9 -4.5 -1.4 -1.7
formate no stress control Formate no stress control +0.1 -5.8 -2.4 -1.8 -1.8
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.6 -2.4 -2.8 -3.8 -2.0
hydrogen no stress control H2 no stress control -0.3 -4.1 -2.2 -2.5 -2.7
nitrogen source Formate N-free with 2mM Adenosine +0.1 -3.7 -2.8 -4.2 -1.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE -0.2 -3.2 -3.5 -3.5 -1.4
stress McCas_formate_complete sulfate 125 mM -1.4 -4.3 -2.9 -1.5 -1.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.0 -3.3 -3.6 -2.8 -1.9
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.4 -3.1 -3.4 -3.2 -1.4
nutrient t1 formate McAA pyroxidine HCl dropout +0.3 -4.7 -3.0 -1.7 -2.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +1.1 -2.4 -3.4 -4.1 -2.4
biofilm Formate pooled triplicate biofilm cells -1.3 -4.0 -2.0 -2.5 -1.4
stress McCas_formate_complete hypophosphite 62.5 mM +0.7 -4.9 -3.5 -0.4 -2.4
nutrient t1 formate McAA lipolic dropout +0.5 -3.4 -2.9 -1.9 -2.6
nitrogen source H2 N-free with 10mM D-glucosamine +0.6 -3.6 -3.2 -2.2 -1.6
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