Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS04950 and MMJJ_RS04955 are separated by 21 nucleotidesMMJJ_RS04955 and MMJJ_RS04960 are separated by 85 nucleotidesMMJJ_RS04960 and MMJJ_RS04965 are separated by 48 nucleotidesMMJJ_RS04965 and MMJJ_RS04970 are separated by 100 nucleotides MMJJ_RS04950: MMJJ_RS04950 - TIGR00375 family protein, at 919,761 to 920,942 _RS04950 MMJJ_RS04955: MMJJ_RS04955 - tRNA pseudouridine(54/55) synthase Pus10, at 920,964 to 922,376 _RS04955 MMJJ_RS04960: MMJJ_RS04960 - 50S ribosomal protein L21e, at 922,462 to 922,755 _RS04960 MMJJ_RS04965: MMJJ_RS04965 - RNA polymerase Rpb4 family protein, at 922,804 to 923,127 _RS04965 MMJJ_RS04970: MMJJ_RS04970 - DUF655 domain-containing protein, at 923,228 to 923,812 _RS04970
Group Condition MMJJ_RS04950 MMJJ_RS04955 MMJJ_RS04960 MMJJ_RS04965 MMJJ_RS04970
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM -0.1 +0.1 N.D. -2.4 -4.8
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -0.0 -0.1 N.D. -3.1 -3.8
planktonic H2 pooled triplicate planktonic cells -0.8 -0.5 N.D. -1.1 -4.4
attachment assay; attached sample McCas-formate glass attached -0.1 -0.1 N.D. -0.2 -5.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.0 +0.2 N.D. -1.7 -4.3
formate no stress control Formate no stress control +0.2 -0.1 N.D. +0.1 -5.8
biofilm Formate pooled triplicate biofilm cells +0.0 -0.2 N.D. -1.3 -4.0
hydrogen no stress control H2 no stress control +0.1 -0.0 N.D. -1.2 -4.3
stress McCas_formate_complete Irgasan 0.0125 mM +0.2 +0.5 N.D. -2.1 -4.2
biofilm H2 pooled triplicate biofilm cells +0.0 -0.6 N.D. -0.2 -4.5
stress McCas_formate_complete Spiramycin 0.1 mM +0.1 -0.4 N.D. -0.2 -4.8
stress McCas_formate_complete silver chloride 0.125 mM +0.1 -0.5 N.D. -2.6 -2.1
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM -0.2 +0.3 N.D. -1.0 -4.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.0 +0.1 N.D. -0.7 -4.5
formate no stress control Formate no stress control +0.1 -0.1 N.D. -0.4 -4.7
stress McCas_formate_complete Rifampicin 0.25 mM +0.3 +0.7 N.D. -1.2 -4.8
stress McCas_formate_complete deta nonoate 0.025 mM +0.1 -0.1 N.D. -1.7 -3.2
stress McCas_formate_complete sulfate 125 mM -0.2 +1.1 N.D. -1.4 -4.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 +0.2 N.D. -0.5 -4.3
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM +0.2 +1.0 N.D. -1.7 -4.0
stress McCas_formate_complete hypophosphite 62.5 mM +0.2 +0.4 N.D. -2.2 -2.8
stress McCas_formate_complete hypophosphite 62.5 mM +0.0 +0.1 N.D. +0.7 -4.9
stress McCas_formate_complete Fluorophosphate 31.25 mM +0.2 +0.9 N.D. -1.3 -3.9
stress McCas_formate_complete Nitrofurantoin 0.025 mM +0.2 +0.3 N.D. -0.2 -4.3
nutrient t1 formate McAA pyroxidine HCl dropout -0.0 +0.6 N.D. +0.3 -4.7
stress McCas_formate_complete Antimycin A 0.01 mM +0.2 +0.6 N.D. -2.1 -2.6
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM +0.2 +1.0 N.D. -0.2 -4.3
stress McCas_formate_complete 3-nitroxypropanol 0.00208333333333333 mM +0.3 +0.9 N.D. -1.7 -2.5
nitrogen source Formate N-free with 2mM Adenosine +0.1 +1.3 N.D. +0.1 -3.7
nitrogen source Formate N-free with 2mM Adenosine -0.1 +1.1 N.D. +0.3 -3.5
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