Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS04915 and MMJJ_RS04920 are separated by 89 nucleotidesMMJJ_RS04920 and MMJJ_RS04925 are separated by 14 nucleotidesMMJJ_RS04925 and MMJJ_RS04930 are separated by 19 nucleotidesMMJJ_RS04930 and MMJJ_RS04935 are separated by 142 nucleotides MMJJ_RS04915: MMJJ_RS04915 - protein-L-isoaspartate O-methyltransferase, at 913,931 to 914,569 _RS04915 MMJJ_RS04920: MMJJ_RS04920 - CBS domain-containing protein, at 914,659 to 915,144 _RS04920 MMJJ_RS04925: MMJJ_RS04925 - cation:proton antiporter, at 915,159 to 916,337 _RS04925 MMJJ_RS04930: MMJJ_RS04930 - MFS transporter, at 916,357 to 917,508 _RS04930 MMJJ_RS04935: MMJJ_RS04935 - 4Fe-4S binding protein, at 917,651 to 917,848 _RS04935
Group Condition MMJJ_RS04915 MMJJ_RS04920 MMJJ_RS04925 MMJJ_RS04930 MMJJ_RS04935
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -0.2 -0.3 -0.0 +0.6 -3.8
nitrogen source H2 N-free with 10mM D-glucosamine +0.0 +0.3 -0.1 -3.8 +0.1
nitrogen source N2 fixation, H2 media +0.2 -0.1 -0.2 -2.6 -0.3
nitrogen source H2 N-free with 2mM Adenosine -0.0 +0.0 +0.0 -1.2 -1.5
nitrogen source H2 N-free with 10mM D-glucosamine -0.0 +0.1 +0.1 -2.8 -0.0
nitrogen source Formate N-free with 2mM Adenosine +0.3 +0.3 -0.3 -1.0 -1.2
nitrogen source H2 N-free with 10mM D-glucosamine +0.1 -0.2 +0.0 -1.9 +0.1
nitrogen source Formate N-free with 2mM Adenosine +0.2 +0.6 +0.1 -1.0 -1.6
stress McCas_formate_complete Spiramycin 0.1 mM +0.0 +0.2 -0.4 -0.5 -1.0
nitrogen source Formate N-free with 2mM Adenosine +0.3 +0.2 +0.1 -1.1 -1.2
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM -0.2 -0.2 -0.2 +0.2 -1.2
stress McCas_formate_complete sodium bromide 125 mM -0.2 -0.3 -0.0 -0.4 -0.7
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.0 -0.1 -0.1 -0.1 -1.2
stress McCas_formate_complete Nitrofurantoin 0.025 mM -0.4 +0.2 -0.0 +0.2 -1.3
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM -0.1 -0.1 -0.3 +0.1 -0.9
no stress control McCas_formate_complete no stress control -0.4 -0.5 -0.3 -0.4 +0.4
stress McCas_formate_complete Nitrofurantoin 0.025 mM +0.3 -0.3 -0.1 +0.1 -0.9
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.1 +0.2 +0.2 -0.1 -1.3
attachment assay; attached sample McCas-formate glass attached +0.2 -0.0 +0.0 +0.1 -1.2
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM +0.4 -0.3 -0.2 +0.1 -0.9
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.1 +0.2 +0.2 -0.2 -1.0
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM +0.0 -0.1 -0.7 +0.6 -0.4
planktonic H2 pooled triplicate planktonic cells +0.2 +0.1 +0.7 +0.0 -1.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.2 +0.2 +0.2 -0.2 -0.7
stress McCas_formate_complete Anhydrotetracycline 0.05 mM +0.0 -0.6 -0.2 -0.1 +0.8
stress McCas_formate_complete hypophosphite 62.5 mM -0.1 +0.1 -0.1 -0.4 +0.6
nitrogen source H2 N-free with 2mM Adenosine +0.3 +0.0 -0.2 -0.4 +0.9
stress McCas_formate_complete Chlorpromazine 0.0166666666666667 mM +0.2 -0.2 -0.5 +0.1 +1.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.1 +0.0 -0.1 +0.3 +0.9
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.1 +0.4 +0.2 -0.3 +0.8
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