Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS04795 and MMJJ_RS04800 are separated by 46 nucleotidesMMJJ_RS04800 and MMJJ_RS04805 are separated by 23 nucleotidesMMJJ_RS04805 and MMJJ_RS04810 are separated by 133 nucleotidesMMJJ_RS04810 and MMJJ_RS04815 are separated by 168 nucleotides MMJJ_RS04795: MMJJ_RS04795 - MBL fold metallo-hydrolase, at 899,071 to 899,688 _RS04795 MMJJ_RS04800: MMJJ_RS04800 - 2-phospho-L-lactate guanylyltransferase, at 899,735 to 900,403 _RS04800 MMJJ_RS04805: MMJJ_RS04805 - N-acetyl-gamma-glutamyl-phosphate reductase, at 900,427 to 901,458 _RS04805 MMJJ_RS04810: MMJJ_RS04810 - nitroreductase family protein, at 901,592 to 902,113 _RS04810 MMJJ_RS04815: MMJJ_RS04815 - TIM barrel protein, at 902,282 to 902,938 _RS04815
Group Condition MMJJ_RS04795 MMJJ_RS04800 MMJJ_RS04805 MMJJ_RS04810 MMJJ_RS04815
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.0 -5.3 N.D. +0.0 -0.3
planktonic H2 pooled triplicate planktonic cells -0.1 -4.9 N.D. +0.1 -0.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.1 -4.8 N.D. +0.1 -0.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 -5.0 N.D. +0.1 -0.2
biofilm H2 pooled triplicate biofilm cells -0.2 -4.7 N.D. +0.0 -0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.0 -5.1 N.D. +0.2 -0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.1 -5.0 N.D. +0.2 -0.1
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.0 -4.6 N.D. +0.2 -0.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE +0.0 -4.6 N.D. +0.3 -0.7
iron Iron excess (2.5x) , H2 media -0.0 -4.8 N.D. +0.1 -0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.1 -4.8 N.D. +0.3 -0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 -4.5 N.D. -0.1 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -4.3 N.D. -0.0 -0.3
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.0 -4.4 N.D. -0.1 -0.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.1 -4.4 N.D. +0.1 -0.2
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 +0.1 -4.7 N.D. +0.0 -0.0
iron control, 1x iron with casaminos and vitamins, H2 media +0.1 -4.2 N.D. -0.0 -0.3
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride +0.1 -4.2 N.D. +0.1 -0.3
stress McCas_formate_complete Sulfaquinoxaline 0.1 mM -0.4 -3.8 N.D. +0.3 -0.3
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.2 -3.8 N.D. -0.4 +0.3
nutrient t1 formate McAA biotin dropout -0.1 -4.2 N.D. -0.0 +0.3
nitrogen source Formate N-free with 2mM Adenosine +0.2 -3.3 N.D. -0.4 -0.6
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.1 -3.6 N.D. +0.1 -0.6
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.2 -3.6 N.D. -0.1 -0.4
nutrient t1 formate McAA folic dropout -0.0 -3.8 N.D. -0.3 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE +0.2 -3.4 N.D. +0.4 -1.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.1 -3.8 N.D. -0.3 +0.4
stress McCas_formate_complete 3-nitroxypropanol 0.00078125 mM -0.0 -4.0 N.D. +0.3 +0.2
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM -0.3 -2.9 N.D. -0.6 +0.5
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -0.1 -3.5 N.D. -0.2 +0.5
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