Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS04660 and MMJJ_RS04665 are separated by 15 nucleotidesMMJJ_RS04665 and MMJJ_RS04670 are separated by 27 nucleotidesMMJJ_RS04670 and MMJJ_RS04675 are separated by 18 nucleotidesMMJJ_RS04675 and MMJJ_RS04680 are separated by 29 nucleotides MMJJ_RS04660: MMJJ_RS04660 - hypoxanthine/guanine phosphoribosyltransferase, at 870,231 to 870,788 _RS04660 MMJJ_RS04665: MMJJ_RS04665 - fumarylacetoacetate hydrolase family protein, at 870,804 to 871,412 _RS04665 MMJJ_RS04670: MMJJ_RS04670 - DUF2117 domain-containing protein, at 871,440 to 872,555 _RS04670 MMJJ_RS04675: MMJJ_RS04675 - thiamine pyrophosphate-binding protein, at 872,574 to 874,079 _RS04675 MMJJ_RS04680: MMJJ_RS04680 - topoisomerase DNA-binding C4 zinc finger domain-containing protein, at 874,109 to 874,981 _RS04680
Group Condition MMJJ_RS04660 MMJJ_RS04665 MMJJ_RS04670 MMJJ_RS04675 MMJJ_RS04680
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.0 +0.0 -2.4 -3.5 -3.8
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.1 -0.0 -2.6 -3.2 -3.8
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.1 -0.2 -2.3 -3.2 -4.0
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.0 -0.1 -2.6 -3.0 -3.7
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -1.3 -0.6 -1.4 -3.0 -3.1
iron Iron excess (2.5x) , H2 media -0.4 -0.2 -2.9 -3.8 -1.9
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.2 +0.1 -2.1 -3.0 -3.8
iron Iron excess (2.5x) , H2 media -0.3 -0.2 -2.6 -3.4 -2.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.0 +0.0 -2.4 -3.0 -3.5
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.1 +0.1 -2.4 -3.2 -3.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.0 -0.1 -2.4 -2.7 -3.5
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.4 +0.0 -2.2 -3.3 -2.8
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.2 -0.1 -1.9 -2.2 -4.0
nitrogen source H2 N-free with 2g/L casaminos -0.4 -0.3 -1.8 -3.9 -2.0
hydrogen no stress control H2 no stress control -0.2 -0.0 -2.6 -3.5 -1.9
iron control, 1x iron with casaminos and vitamins, H2 media -0.3 -0.1 -2.0 -3.5 -2.2
planktonic H2 pooled triplicate planktonic cells -0.8 +0.3 -2.7 -3.0 -1.6
nitrogen source H2 N-free with 10mM D-glucosamine -0.7 +0.1 -2.4 -3.2 -1.5
biofilm H2 pooled triplicate biofilm cells -0.6 +0.1 -2.7 -2.4 -2.0
iron Iron excess (2.5x) , H2 media -0.4 -0.1 -2.6 -3.0 -1.6
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.3 +0.1 -1.2 -4.2 -2.1
hydrogen no stress control H2 no stress control -0.1 -0.1 -2.4 -3.3 -1.7
iron Iron limitation (0.2x), H2 media -0.3 -0.1 -2.2 -3.2 -1.6
nitrogen source H2 N-free with 10mM Ammonium chloride -0.4 -0.2 -2.2 -3.3 -1.3
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.4 +0.0 -2.0 -3.0 -2.1
iron Iron limitation (0.2x), H2 media -0.3 -0.1 -1.6 -3.6 -1.7
nitrogen source H2 N-free with 2g/L casaminos -0.4 +0.1 -2.3 -3.0 -1.7
nitrogen source Formate N-free with 2mM Adenosine +3.4 -0.4 -2.0 -3.4 -2.3
nitrogen source Formate N-free with 2mM Adenosine +3.6 -0.1 -1.7 -2.4 -1.7
nitrogen source Formate N-free with 2mM Adenosine +3.8 -0.2 -1.4 -2.2 -1.4
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