Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS04105 and MMJJ_RS04110 are separated by 13 nucleotidesMMJJ_RS04110 and MMJJ_RS04115 are separated by 81 nucleotidesMMJJ_RS04115 and MMJJ_RS04120 are separated by 40 nucleotidesMMJJ_RS04120 and MMJJ_RS04125 are separated by 28 nucleotides MMJJ_RS04105: MMJJ_RS04105 - prepilin peptidase, at 761,839 to 762,879 _RS04105 MMJJ_RS04110: MMJJ_RS04110 - hypothetical protein, at 762,893 to 763,261 _RS04110 MMJJ_RS04115: MMJJ_RS04115 - HDIG domain-containing protein, at 763,343 to 764,029 _RS04115 MMJJ_RS04120: MMJJ_RS04120 - amino acid ABC transporter ATP-binding protein, at 764,070 to 764,801 _RS04120 MMJJ_RS04125: MMJJ_RS04125 - tRNA (guanine(10)-N(2))-dimethyltransferase, at 764,830 to 765,951 _RS04125
Group Condition MMJJ_RS04105 MMJJ_RS04110 MMJJ_RS04115 MMJJ_RS04120 MMJJ_RS04125
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.1 -1.1 +0.2 +0.0 -2.6
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.1 -1.0 +0.2 -0.1 -2.5
nutrient t1 formate McAA folic dropout +0.4 -2.2 -0.2 -0.1 -1.5
nutrient t1 formate McAA pantothenate dropout +0.3 -2.0 -0.2 +0.1 -1.8
iron Iron limitation (0.2x), formate media +0.4 -2.3 -0.1 -0.1 -1.3
nutrient t1 formate McAA biotin dropout +0.3 -1.8 -0.3 -0.2 -1.5
stress Formate 50 mM hypophosphite +0.3 -2.9 -0.1 +0.0 -0.7
nutrient t1 formate McAA folic dropout +0.5 -2.0 -0.1 -0.1 -1.6
iron Iron limitation (0.2x), formate media +0.5 -2.3 -0.0 -0.1 -1.3
nutrient t1 formate McAA pyroxidine HCl dropout +0.4 -2.0 -0.2 +0.0 -1.5
iron control, 1x iron with casaminos and vitamins, H2 media +0.3 -3.1 -0.1 +0.2 -0.4
nutrient t1 formate McAA riboflavin dropout +0.4 -2.0 -0.1 +0.2 -1.6
nutrient t1 formate McAA lipolic dropout +0.4 -2.0 +0.1 -0.1 -1.4
iron control, 1x iron with casaminos and vitamins, formate media +0.6 -1.9 -0.1 -0.0 -1.6
nitrogen source formate N-free with 10mM Ammonium chloride +0.5 -2.2 -0.2 -0.1 -1.0
nutrient t1 formate McAA nicotinic dropout +0.4 -2.1 -0.2 +0.1 -1.2
iron Iron excess (2.5x) , formate media +0.5 -2.1 -0.1 +0.0 -1.4
iron control, 1x iron with casaminos and vitamins, formate media +0.5 -1.8 -0.3 +0.1 -1.4
nitrogen source H2 N-free with 10mM Ammonium chloride +0.6 -3.2 -0.2 +0.0 -0.2
nitrogen source H2 N-free with 10mM Ammonium chloride +0.6 -3.1 -0.2 -0.2 +0.0
nitrogen source H2 N-free with 10mM Ammonium chloride +0.5 -3.3 +0.1 -0.0 -0.1
planktonic H2 pooled triplicate planktonic cells +0.2 -3.3 +0.4 +0.0 -0.1
iron control, 1x iron with casaminos and vitamins, H2 media +0.4 -3.2 -0.0 +0.2 -0.2
iron Iron excess (2.5x) , formate media +0.5 -2.0 -0.1 +0.1 -1.2
iron Iron excess (2.5x) , H2 media +0.8 -2.6 +0.0 +0.2 -0.3
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM +0.5 -1.8 +0.4 +0.3 -1.2
nitrogen source Formate N-free with 2mM Adenosine +0.8 -2.6 -0.1 -0.1 +0.3
stress McCas_formate_complete Rifampicin 0.25 mM +0.7 -1.5 +0.1 +0.5 -1.1
stress McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM +0.9 -1.2 +0.4 +0.3 -1.3
nitrogen source N2 fixation, H2 media +0.7 -1.6 +0.3 +0.2 +1.2
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