Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS04030 and MMJJ_RS04035 are separated by 7 nucleotidesMMJJ_RS04035 and MMJJ_RS04040 are separated by 57 nucleotidesMMJJ_RS04040 and MMJJ_RS04045 are separated by 37 nucleotidesMMJJ_RS04045 and MMJJ_RS04050 overlap by 8 nucleotides MMJJ_RS04030: MMJJ_RS04030 - rRNA maturation protein, at 750,846 to 751,340 _RS04030 MMJJ_RS04035: MMJJ_RS04035 - hypothetical protein, at 751,348 to 751,599 _RS04035 MMJJ_RS04040: MMJJ_RS04040 - prefoldin subunit beta, at 751,657 to 751,998 _RS04040 MMJJ_RS04045: MMJJ_RS04045 - DHH family phosphoesterase, at 752,036 to 753,052 _RS04045 MMJJ_RS04050: MMJJ_RS04050 - dihydroneopterin aldolase family protein, at 753,045 to 753,419 _RS04050
Group Condition MMJJ_RS04030 MMJJ_RS04035 MMJJ_RS04040 MMJJ_RS04045 MMJJ_RS04050
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 N.D. N.D. -6.2 -3.4 -3.8
nitrogen source H2 N-free with 2mM Adenosine N.D. N.D. -2.5 -1.7 -3.6
nitrogen source H2 N-free with 2g/L casaminos N.D. N.D. -1.3 -0.8 -5.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. N.D. -1.6 -1.4 -4.4
nutrient t1 formate McAA folic dropout N.D. N.D. -0.8 -0.5 -6.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. N.D. -1.5 -1.2 -4.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE N.D. N.D. -1.5 -1.2 -4.4
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. N.D. -0.7 -0.8 -5.4
attachment assay; attached sample McCas-formate low carbon steel attached N.D. N.D. -0.4 -0.1 -6.3
biofilm H2 pooled triplicate biofilm cells N.D. N.D. -2.1 -0.7 -3.9
iron Iron excess (2.5x) , H2 media N.D. N.D. -1.4 -0.9 -4.3
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE N.D. N.D. -0.8 -1.0 -4.5
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. N.D. -1.0 -0.8 -4.4
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. N.D. -1.2 -0.7 -4.3
nutrient t1 formate McAA B12 dropout N.D. N.D. -0.8 -0.8 -4.5
nitrogen source H2 N-free with 10mM Ammonium chloride N.D. N.D. -1.0 -0.6 -4.4
iron control, 1x iron with casaminos and vitamins, H2 media N.D. N.D. -1.2 -0.7 -4.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE N.D. N.D. -0.4 -1.3 -4.3
iron control, 1x iron with casaminos and vitamins, H2 media N.D. N.D. -1.2 -0.6 -4.1
nutrient t1 formate McAA thiamine dropout N.D. N.D. -0.6 -0.9 -4.4
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE N.D. N.D. -0.4 -1.0 -4.5
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride N.D. N.D. -0.8 -0.8 -4.2
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. N.D. -1.5 -1.3 -2.8
nutrient t1 formate vitamin complete N.D. N.D. -0.6 -0.5 -4.5
iron control, 1x iron with casaminos and vitamins, H2 media N.D. N.D. -0.8 -0.6 -4.1
stress McCas_formate_complete Spiramycin 0.1 mM N.D. N.D. -1.1 -1.0 -3.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE N.D. N.D. -0.7 -0.6 -4.2
nutrient t1 formate McAA pyroxidine HCl dropout N.D. N.D. -0.7 -0.3 -4.4
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE N.D. N.D. -0.7 -0.7 -4.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE N.D. N.D. +0.2 -1.3 -4.2
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