Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS03870 and MMJJ_RS03875 are separated by 12 nucleotidesMMJJ_RS03875 and MMJJ_RS03880 are separated by 75 nucleotidesMMJJ_RS03880 and MMJJ_RS03885 are separated by 129 nucleotidesMMJJ_RS03885 and MMJJ_RS03890 are separated by 24 nucleotides MMJJ_RS03870: MMJJ_RS03870 - beta-ribofuranosylaminobenzene 5'-phosphate synthase, at 720,721 to 721,698 _RS03870 MMJJ_RS03875: MMJJ_RS03875 - CBS domain-containing protein, at 721,711 to 722,172 _RS03875 MMJJ_RS03880: MMJJ_RS03880 - TraB/GumN family protein, at 722,248 to 723,390 _RS03880 MMJJ_RS03885: MMJJ_RS03885 - tetratricopeptide repeat protein, at 723,520 to 724,554 _RS03885 MMJJ_RS03890: MMJJ_RS03890 - right-handed parallel beta-helix repeat-containing protein, at 724,579 to 727,356 _RS03890
Group Condition MMJJ_RS03870 MMJJ_RS03875 MMJJ_RS03880 MMJJ_RS03885 MMJJ_RS03890
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 N.D. -3.6 -3.3 +0.1 -0.1
nitrogen source Formate N-free with 2mM Adenosine N.D. -3.6 -3.0 -0.2 -0.0
nitrogen source Formate N-free with 2mM Adenosine N.D. -3.6 -2.5 -0.1 +0.0
nitrogen source Formate N-free with 2mM Adenosine N.D. -3.3 -2.0 -0.2 -0.0
nutrient t1 formate McAA B12 dropout N.D. -4.8 -0.9 +0.0 +0.1
nutrient t1 formate McAA folic dropout N.D. -4.6 -0.9 +0.0 +0.0
stress McCas_formate_complete Rifampicin 0.25 mM N.D. -3.4 -1.6 -0.0 -0.2
nutrient t1 formate McAA biotin dropout N.D. -4.3 -0.9 -0.1 +0.1
iron control, 1x iron with casaminos and vitamins, formate media N.D. -4.3 -0.8 -0.1 -0.0
nutrient t1 formate vitamin complete N.D. -4.1 -0.9 -0.1 -0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE N.D. -3.1 -2.0 +0.1 -0.0
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 N.D. -3.7 -1.2 +0.1 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE N.D. -3.2 -2.0 +0.2 +0.1
nutrient t1 formate McAA folic dropout N.D. -3.9 -1.0 -0.0 +0.0
iron Iron limitation (0.2x), formate media N.D. -3.9 -1.0 -0.0 +0.0
nutrient t1 formate McAA thiamine dropout N.D. -3.7 -1.0 -0.1 +0.1
stress McCas_formate_complete hypophosphite 62.5 mM N.D. -4.0 -0.6 -0.1 -0.0
stress McCas_formate_complete deta nonoate 0.025 mM N.D. -3.1 -1.7 +0.0 +0.0
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE N.D. -2.6 -2.2 +0.1 -0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE N.D. -3.2 -1.3 -0.2 -0.0
stress McCas_formate_complete 3-nitroxypropanol 0.00078125 mM N.D. -3.6 -1.1 -0.0 +0.0
iron control, 1x iron with casaminos and vitamins, formate media N.D. -3.8 -0.7 +0.0 -0.1
stress McCas_formate_complete Chlorite 0.3125 mM N.D. -3.4 -1.2 -0.0 -0.0
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE N.D. -3.3 -1.4 +0.1 +0.0
nutrient t1 formate McAA nicotinic dropout N.D. -3.8 -0.8 +0.0 -0.0
iron control, 1x iron with casaminos and vitamins, H2 media N.D. -4.3 -0.3 +0.0 +0.1
nutrient t1 formate McAA pantothenate dropout N.D. -3.7 -0.8 -0.0 +0.1
nutrient t1 formate McAA p-aminobenzoic dropout N.D. -3.5 -0.9 -0.1 +0.1
stress Formate 100 mM hypophosphite N.D. -3.7 -0.7 +0.1 +0.0
nutrient t1 formate McAA lipolic dropout N.D. -3.5 -0.9 +0.1 +0.1
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