Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS03595 and MMJJ_RS03600 are separated by 70 nucleotidesMMJJ_RS03600 and MMJJ_RS03605 are separated by 161 nucleotidesMMJJ_RS03605 and MMJJ_RS03610 are separated by 436 nucleotidesMMJJ_RS03610 and MMJJ_RS03615 are separated by 1 nucleotides MMJJ_RS03595: MMJJ_RS03595 - hypothetical protein, at 670,823 to 671,236 _RS03595 MMJJ_RS03600: MMJJ_RS03600 - TrmJ/YjtD family RNA methyltransferase, at 671,307 to 672,011 _RS03600 MMJJ_RS03605: MMJJ_RS03605 - TIGR00297 family protein, at 672,173 to 672,886 _RS03605 MMJJ_RS03610: MMJJ_RS03610 - HIT family protein, at 673,323 to 673,715 _RS03610 MMJJ_RS03615: MMJJ_RS03615 - cobalt-precorrin-8 methylmutase, at 673,717 to 674,349 _RS03615
Group Condition MMJJ_RS03595 MMJJ_RS03600 MMJJ_RS03605 MMJJ_RS03610 MMJJ_RS03615
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.6 -0.3 -0.8 -0.2 -5.2
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.5 -0.3 -0.7 -0.1 -5.1
iron Iron limitation (0.2x), H2 media -0.3 -0.2 -0.2 -0.5 -5.3
iron control, 1x iron with casaminos and vitamins, H2 media -0.6 -0.4 -0.3 -0.1 -5.1
iron control, 1x iron with casaminos and vitamins, H2 media -0.7 -0.4 -0.5 -0.4 -4.5
iron Iron limitation (0.2x), H2 media -0.3 -0.2 -0.5 -0.7 -4.6
iron Iron excess (2.5x) , H2 media -0.5 -0.0 -0.9 -0.2 -4.6
nitrogen source H2 N-free with 2g/L casaminos -0.4 -0.4 -0.4 -0.2 -4.7
nitrogen source H2 N-free with 2g/L casaminos -0.3 -0.3 -1.0 +0.1 -4.6
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -0.5 -1.6 -0.1 -0.5 -3.4
nitrogen source H2 N-free with 10mM Ammonium chloride -0.7 -0.3 -1.0 +0.3 -4.3
nitrogen source H2 N-free with 10mM Ammonium chloride -0.5 -0.2 -0.3 +0.2 -5.1
nitrogen source H2 N-free with 10mM Ammonium chloride -0.6 -0.2 -0.1 +0.1 -5.1
iron Iron limitation (0.2x), H2 media -0.3 -0.4 -0.2 -0.3 -4.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.7 -0.5 +0.2 -0.8 -4.1
nitrogen source H2 N-free with 2g/L casaminos -0.3 -0.3 -1.0 -0.2 -4.0
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.5 -0.1 +0.1 -0.1 -4.7
biofilm H2 pooled triplicate biofilm cells -0.3 +0.2 -1.2 -0.5 -3.6
iron control, 1x iron with casaminos and vitamins, formate media +0.2 -0.4 +0.2 -0.3 -5.1
nutrient t1 formate McAA riboflavin dropout +0.1 -0.4 +0.2 +0.1 -5.4
nutrient t1 formate McAA riboflavin dropout -0.1 -0.3 +0.4 +0.1 -5.3
attachment assay; attached sample McCas-formate glass attached +0.1 +0.0 -0.7 +0.2 -4.6
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.9 -0.3 +0.6 -0.5 -3.9
nitrogen source Formate N-free with 2mM Adenosine -0.8 -0.6 +0.4 -0.2 -3.7
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.5 -1.0 +0.3 +0.3 -3.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.6 -0.5 +0.8 -0.2 -4.0
nutrient t1 formate McAA B12 dropout +0.3 -0.4 +0.7 +0.3 -4.8
nutrient t1 formate McAA B12 dropout +0.4 -0.6 +0.6 -0.0 -4.1
nitrogen source H2 N-free with 10mM D-glucosamine -0.4 -0.3 +0.8 +0.6 -4.5
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.9 -0.5 +1.6 -0.4 -3.7
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