Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS03185 and MMJJ_RS03190 are separated by 199 nucleotidesMMJJ_RS03190 and MMJJ_RS03195 are separated by 241 nucleotidesMMJJ_RS03195 and MMJJ_RS03200 are separated by 15 nucleotidesMMJJ_RS03200 and MMJJ_RS03205 are separated by 58 nucleotides MMJJ_RS03185: MMJJ_RS03185 - alanine--tRNA ligase, at 601,763 to 604,441 _RS03185 MMJJ_RS03190: MMJJ_RS03190 - phosphopyruvate hydratase, at 604,641 to 605,933 _RS03190 MMJJ_RS03195: MMJJ_RS03195 - hypothetical protein, at 606,175 to 606,453 _RS03195 MMJJ_RS03200: MMJJ_RS03200 - uroporphyrinogen-III synthase, at 606,469 to 607,203 _RS03200 MMJJ_RS03205: MMJJ_RS03205 - metallophosphoesterase, at 607,262 to 607,777 _RS03205
Group Condition MMJJ_RS03185 MMJJ_RS03190 MMJJ_RS03195 MMJJ_RS03200 MMJJ_RS03205
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -2.4 -2.7 -1.8 -3.4 -1.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -1.8 -2.8 -1.9 -3.4 -1.0
stress McCas_formate_complete deta nonoate 0.025 mM -1.5 -3.1 -1.7 -3.7 -0.7
stress McCas_formate_complete Polymyxin B sulfate 0.05 mM -2.0 -3.2 -0.6 -1.7 -3.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE -1.8 -2.8 -1.9 -3.0 -1.1
stress McCas_formate_complete Nitrofurantoin 0.025 mM -3.5 -1.2 -2.2 -1.9 -1.2
stress McCas_formate_complete Ticarcillin 12.5 mM -2.6 -1.9 -1.9 -2.7 -0.5
stress McCas_formate_complete Spiramycin 0.1 mM -1.9 -3.2 -1.2 -3.3 -0.0
nutrient t1 formate McAA pyroxidine HCl dropout -0.5 -4.2 -1.0 -3.4 -0.5
nutrient t1 formate vitamin complete -1.2 -3.2 -1.0 -3.7 -0.1
no stress control McCas_formate_complete no stress control -2.8 -1.9 -1.7 -2.1 -0.6
stress McCas_formate_complete Irgasan 0.0125 mM -3.0 -2.4 -0.8 -2.5 -0.4
stress McCas_formate_complete sodium nitrite 0.416666666666667 mM -3.7 -1.9 -1.2 -1.0 -1.1
stress McCas_formate_complete sodium nitrite 0.125 mM -0.5 -3.4 -1.5 -3.6 +0.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -0.7 -3.2 -1.3 -3.8 +0.3
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.3 -3.6 -0.9 -3.9 -0.4
hydrogen no stress control H2 no stress control -0.1 -3.9 -0.6 -4.1 +0.3
hydrogen no stress control H2 no stress control +0.3 -3.5 -0.4 -4.8 +0.2
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -1.2 -2.9 -1.7 -3.6 +1.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -1.4 -2.9 -1.3 -3.6 +1.2
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE +0.9 -3.2 -0.8 -3.8 -0.9
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -1.7 -2.8 -2.8 -3.5 +3.0
planktonic H2 pooled triplicate planktonic cells +1.2 -3.4 -2.2 -3.1 -0.1
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.6 -3.8 -0.4 -4.0 +0.7
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE +0.1 -3.7 -0.3 -4.0 +1.3
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.7 -3.9 -0.3 -4.2 +1.0
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -0.3 -3.4 +0.1 -4.2 +1.4
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 +0.3 -3.7 -0.2 -4.0 +1.3
nitrogen source H2 N-free with 2g/L casaminos +0.0 -3.6 +0.5 -4.1 +1.4
iron Iron excess (2.5x) , H2 media +0.8 -3.0 -0.3 -4.0 +1.0
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