Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS03160 and MMJJ_RS03165 are separated by 18 nucleotidesMMJJ_RS03165 and MMJJ_RS03170 are separated by 37 nucleotidesMMJJ_RS03170 and MMJJ_RS03175 are separated by 9 nucleotidesMMJJ_RS03175 and MMJJ_RS03180 are separated by 10 nucleotides MMJJ_RS03160: MMJJ_RS03160 - winged helix-turn-helix transcriptional regulator, at 596,958 to 598,319 _RS03160 MMJJ_RS03165: MMJJ_RS03165 - methionine synthase, at 598,338 to 599,276 _RS03165 MMJJ_RS03170: MMJJ_RS03170 - DUF5612 domain-containing protein, at 599,314 to 599,967 _RS03170 MMJJ_RS03175: MMJJ_RS03175 - zinc metalloprotease HtpX, at 599,977 to 600,834 _RS03175 MMJJ_RS03180: MMJJ_RS03180 - pantoate kinase, at 600,845 to 601,720 _RS03180
Group Condition MMJJ_RS03160 MMJJ_RS03165 MMJJ_RS03170 MMJJ_RS03175 MMJJ_RS03180
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -1.2 N.D. -5.7 -0.3 N.D.
nitrogen source H2 N-free with 10mM D-glucosamine -3.2 N.D. -3.5 -0.4 N.D.
nitrogen source H2 N-free with 10mM D-glucosamine -3.0 N.D. -3.4 -0.6 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -1.0 N.D. -5.6 -0.3 N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.7 N.D. -5.5 -0.3 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.5 N.D. -5.7 -0.3 N.D.
iron Iron excess (2.5x) , H2 media -2.0 N.D. -3.9 -0.4 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -1.2 N.D. -4.7 -0.4 N.D.
nitrogen source H2 N-free with 10mM Ammonium chloride -1.9 N.D. -3.8 -0.5 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.4 N.D. -5.2 -0.3 N.D.
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -1.8 N.D. -3.6 -0.6 N.D.
iron Iron excess (2.5x) , H2 media -2.1 N.D. -3.0 -0.7 N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE -0.9 N.D. -4.5 -0.3 N.D.
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -2.3 N.D. -2.9 -0.4 N.D.
nutrient t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE -0.7 N.D. -4.8 -0.2 N.D.
iron Iron limitation (0.2x), H2 media -2.1 N.D. -2.7 -0.8 N.D.
nitrogen source H2 N-free with 2g/L casaminos -2.0 N.D. -3.0 -0.6 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE -0.7 N.D. -4.7 +0.0 N.D.
nitrogen source H2 N-free with 2g/L casaminos 10mM Ammonium chloride -1.9 N.D. -3.1 -0.4 N.D.
iron control, 1x iron with casaminos and vitamins, H2 media -1.7 N.D. -3.3 -0.4 N.D.
rich t2 H2 McCas, 5g/L bicarb, 40 psi H2CO2 -1.5 N.D. -4.8 +0.9 N.D.
stress Formate 50 mM hypophosphite -3.3 N.D. -1.5 -0.5 N.D.
iron control, 1x iron with casaminos and vitamins, H2 media -1.6 N.D. -3.5 -0.2 N.D.
rich t2 H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 -0.5 N.D. -4.9 +0.2 N.D.
stress McCas_formate_complete Spiramycin 0.1 mM -2.3 N.D. -2.8 -0.2 N.D.
biofilm Formate pooled triplicate biofilm cells -0.5 N.D. -3.1 -1.6 N.D.
iron Iron limitation (0.2x), H2 media -2.3 N.D. -2.6 -0.4 N.D.
stress Formate 50 mM hypophosphite -2.6 N.D. -1.9 -0.7 N.D.
stress McCas_formate_complete Rifampicin 0.25 mM -2.6 N.D. -2.5 +0.2 N.D.
stress McCas_formate_complete Rifampicin 0.25 mM -2.8 N.D. -2.2 +0.1 N.D.
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