Fitness for 5 genes in Methanococcus maripaludis JJ

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500 ntMMJJ_RS02170 and MMJJ_RS02175 are separated by 9 nucleotidesMMJJ_RS02175 and MMJJ_RS02180 are separated by 75 nucleotidesMMJJ_RS02180 and MMJJ_RS02185 are separated by 61 nucleotidesMMJJ_RS02185 and MMJJ_RS02190 are separated by 216 nucleotides MMJJ_RS02170: MMJJ_RS02170 - 2-thiouracil desulfurase family protein, at 412,038 to 412,586 _RS02170 MMJJ_RS02175: MMJJ_RS02175 - imidazoleglycerol-phosphate dehydratase HisB, at 412,596 to 413,168 _RS02175 MMJJ_RS02180: MMJJ_RS02180 - DHH family phosphoesterase, at 413,244 to 414,539 _RS02180 MMJJ_RS02185: MMJJ_RS02185 - TIGR00296 family protein, at 414,601 to 415,212 _RS02185 MMJJ_RS02190: MMJJ_RS02190 - molybdopterin biosynthesis protein, at 415,429 to 417,279 _RS02190
Group Condition MMJJ_RS02170 MMJJ_RS02175 MMJJ_RS02180 MMJJ_RS02185 MMJJ_RS02190
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.0 N.D. +0.1 +0.1 -4.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.1 N.D. +0.1 +0.1 -4.1
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.0 N.D. +0.1 -0.0 -3.9
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE +0.0 N.D. +0.1 +0.2 -3.9
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE -0.0 N.D. +0.1 +0.1 -3.8
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE +0.2 N.D. +0.1 -0.0 -3.6
iron Iron excess (2.5x) , H2 media -0.4 N.D. -0.9 -0.0 -2.0
nitrogen source Formate N-free with 2mM Adenosine +0.1 N.D. -0.1 +0.1 -3.3
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.1 N.D. -0.1 +0.1 -3.0
planktonic H2 pooled triplicate planktonic cells +0.1 N.D. -0.5 +0.1 -2.7
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE -0.1 N.D. -0.1 +0.1 -2.7
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE -0.1 N.D. +0.2 +0.1 -2.9
stress Formate 100 mM hypophosphite +0.1 N.D. +0.0 +0.1 -2.8
nitrogen source Formate N-free with 2mM Adenosine +0.4 N.D. +0.2 -0.0 -3.3
nitrogen source Formate N-free with 2mM Adenosine +0.7 N.D. +0.2 -0.2 -3.3
nitrogen source H2 N-free with 2mM Adenosine +0.6 N.D. -0.7 -0.2 -2.3
rich t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE +0.2 N.D. +0.0 +0.0 -2.7
stress Formate 100 mM hypophosphite +0.1 N.D. -0.0 +0.1 -2.6
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.0 N.D. +0.4 +0.1 -2.8
nutrient t2 Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE +0.1 N.D. +0.3 -0.0 -2.6
nitrogen source H2 N-free with 2mM Adenosine +0.2 N.D. -0.6 +0.1 -1.7
stress McCas_formate_complete selenate 31.25 mM -0.2 N.D. +0.1 +0.4 -2.1
nutrient t1 formate McAA B12 dropout +0.2 N.D. +0.4 +0.3 -2.0
stress McCas_formate_complete Fluorophosphate 31.25 mM -0.4 N.D. -0.4 +0.6 +1.6
stress McCas_formate_complete Nitrofurantoin 0.025 mM +0.4 N.D. -0.5 +0.2 +1.6
stress McCas_formate_complete sulfate 125 mM +0.3 N.D. -0.0 +0.6 +2.1
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM +0.0 N.D. +0.4 -0.3 +2.8
nitrogen source N2 fixation, H2 media +0.2 N.D. +0.0 +0.1 +3.9
nitrogen source H2 N-free with 10mM D-glucosamine +0.1 N.D. -0.3 +0.2 +4.1
stress McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM +0.4 N.D. +0.3 +0.3 +3.2
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